Allergy Methods and Protocols

by Jones, Meinir G.; Lympany, Penny

• ISBN13: 9780896038967
• ISBN10: 0896038963
• Edition: 1st
• Format: Hardcover
• Copyright: 2008-06-30
• Publisher: Humana Pr Inc

Summary

Allergy: Mechanisms and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

Table of Contents

Preface	p. v
Contributors	p. ix
Understanding of the Molecular Mechanisms of Allergy	p. 1
T Cell - Primary Culture from Peripheral Blood	p. 17
Production of T-Cell Lines	p. 31
Production of Human T-Cell Clones	p. 43
Mapping of Human T-Cell Epitopes of Allergens	p. 51
Determining MHC Restriction of T-cell Responses	p. 57
Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining	p. 73
Isolation, Flow Cytometric Analysis, and Suppression Assay of CD4+ CD25+ T-Regulatory Cells	p. 85
Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production	p. 97
Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture	p. 107
Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses	p. 121
Standardization of Allergen Extracts	p. 133
Immunoelectrophoresis for the Characterization of Allergen Extracts	p. 147
Conjugation of Haptens	p. 167
Monoclonal Antibodies	p. 183
Purification of Antibodies	p. 197
Collection of Air Samples to Quantify Exposure to Airborne Allergens	p. 209
Assay of Air Sample Eluates	p. 217
The Halogen Assay - A New Technique for Measuring Airborne Allergen	p. 227
Measurement of Specific IgG Anti-Fel d 1 Antibodies	p. 247
The Facilitated Antigen Binding (FAB) Assay - A Protocol to Measure Allergen-Specific Inhibitory Antibody Activity	p. 255
Microscopic Identification and Purity Determination of Pollen Grains	p. 263
Biopanning for the Characterization of Allergen Mimotopes	p. 271
Identification of Mast Cells and Mast Cell Subpopulations	p. 285
Purification and Characterization of Mast Cell Tryptase and Chymase from Human Tissues	p. 299
Experimental Activation of Mast Cells and Their Pharmacological Modulation	p. 319
In situ Hybridization	p. 331
Index	p. 349
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