Basic Bioscience Laboratory Techniques A Pocket Guide

by Bonner, Philip L.R.; Hargreaves, Alan J.

• ISBN13: 9780470743096
• ISBN10: 0470743093
• Edition: 1st
• Format: Paperback
• Copyright: 2011-03-14
• Publisher: Wiley

Summary

This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further readingAll of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations.In short, this is a ls"must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.

Author Biography

Dr Philip L.R. Bonner and Dr Alan J. Hargreaves, both of the School of Science and Technology, Nottingham Trent University.

Table of Contents

Preface	p. ix
Glossary	p. xi
Abbreviations	p. xvii
The preparation of solutions in bioscience research	p. 1
Introduction	p. 1
Concentration	p. 2
Using balances to weigh out reagents	p. 3
Practical considerations when making a 1.0 M solution	p. 6
Dilutions and the use of pipettes	p. 11
Water, acids and bases	p. 15
Buffers	p. 21
The equilibrium/dissociation constant (Ka) for an acid or base and the Henderson-Hasselbalch equation	p. 24
Summary	p. 26
Microscopy	p. 27
Introduction	p. 27
Microscopes - general principles	p. 29
Principles of image formation	p. 30
Light microscopy	p. 32
Electron microscopy	p. 46
Summary	p. 49
Spectrophotometry	p. 51
Introduction	p. 51
The electromagnetic spectrum	p. 51
The absorbance of light	p. 54
Absorbtion spectrophotometry	p. 56
The laws of the absorbance of light	p. 57
The Beer Lambert law	p. 61
Spectrophotometers	p. 63
Applications of spectrophotometry in bioscience	p. 68
Summary	p. 73
Data analysis and presentation	p. 74
Introduction	p. 74
Statistical analysis of data: some key definitions	p. 76
Distributions	p. 79
Statistical comparison of data	p. 85
Presentation, structure and organization of data in laboratory reports	p. 94
Summary	p. 100
The extraction and clarification of biological material	p. 103
General introduction	p. 103
Extraction	p. 103
Extraction methods for animal and plant tissue	p. 105
Extraction methods for bacteria	p. 107
Clarification	p. 108
Centrifugation techniques	p. 115
Points of good practice in centrifugation	p. 119
Summary	p. 120
Electrophoresis of proteins and nucleic acids	p. 121
General introduction	p. 121
Separation of protein mixtures by gel electrophoresis	p. 122
Other electrophoretic techniques applied to proteins	p. 136
Separation of nucleic acids by gel electrophoresis	p. 137
Applications of gel electrophoresis of nucleic acids	p. 141
Summary	p. 146
Chromatography	p. 147
General introduction	p. 147
The theory of chromatography	p. 147
Factors to consider in chromatography	p. 152
Methods used to elute samples in chromatography	p. 153
Different types of chromatography and what properties can be used to separate molecules	p. 153
Thin layer chromatography (TLC)	p. 156
High pressure liquid chromatography (HPLC)	p. 158
Gas liquid chromatography (GLC)	p. 161
Ion exchange chromatography (IEX)	p. 162
Size exclusion chromatography	p. 164
Affinity chromatography	p. 166
Summary	p. 167
Cell culture techniques	p. 169
Introduction	p. 169
Growth and maintenance of cells in culture	p. 174
Summary	p. 192
Suggestions for further reading	p. 195
Index	p. 198
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