Your biochemistry lab course is an essential component in training for a career in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Biochemistry Laboratory: Modern Theory and Techniques covers the theories, techniques, and methodologies practiced in the biochemistry teaching and research lab. Instead of specific experiments, it focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
Rod Boyer served on the faculty at Hope College, Holland, MI, where he taught, researched, and wrote biochemistry for 26 years. He earned his B.A. in chemistry and mathematics at Westmar College (Iowa) and his Ph.D. in physical organic chemistry at Colorado State University. After three years as an NIH Postdoctoral Fellow with M. J. Coon (cytochromes P-450) in the Department of Biological Chemistry at the University of Michigan Medical School, he joined the chemistry faculty at Hope. There he directed the work of over 75 undergraduate students in research supported by the NIH, NSF, Dreyfus Foundation, Howard Hughes Medical Institute, and the Petroleum Research Fund (ACS). With his students, he published numerous journal articles in the areas of ferritin iron storage and biochemical education. He spent a sabbatical year as an American Cancer Society Scholar in the lab of Nobel laureate, Tom Cech at the University of Colorado, Boulder. Rod is also the author of Modern Experimental Biochemistry (Third Edition, 2000, Benjamin-Cummings) and Concepts in Biochemistry (Third Edition, 2006, John Wiley & Sons) and serves as an Associate Editor for the journal Biochemistry and Molecular Biology Education (BAMBED). He is a member of the American Society for Biochemistry and Molecular Biology (ASBMB) and a former member of its Education and Professional Development Committee that recently designed the undergraduate biochemistry degree recommended by the ASBMB. Rod now resides in Bozeman, Montana, where he continues to write and consult in biochemical education.
Table of Contents
Chapter 1: Introduction to the Biochemistry/Molecular Biology Laboratory
Chapter 2: Using the Computer and Internet for Research in Biochemistry
Chapter 3: General Laboratory Procedures
Chapter 4: Centrifugation Techniques in Biochemistry/Molecular Biology
Chapter 5: Purification and Analysis of Biomolecules by Chromatography
Chapter 6: Characterization of Proteins and Nucleic Acids by Electrophoresis
Chapter 7: Spectroscopic Analysis of Biomolecules
Chapter 8: Biomolecular Interactions: Ligand Binding and Enzyme Reacations
Chapter 9: Molecular Biology I: Structures and Analysis of Nucleic Acids
Chapter 10: Molecular Biology II: Recombinant DNA, Molecular Cloning and Enzymology
Chapter 11: Protein Production, Purification, and Characterization