CART

(0) items

Biochemistry Laboratory : Modern Theory and Techniques,9780805346138
This item qualifies for
FREE SHIPPING!

FREE SHIPPING OVER $59!

Your order must be $59 or more, you must select US Postal Service Shipping as your shipping preference, and the "Group my items into as few shipments as possible" option when you place your order.

Bulk sales, PO's, Marketplace Items, eBooks, Apparel, and DVDs not included.

Biochemistry Laboratory : Modern Theory and Techniques

by
Edition:
1st
ISBN13:

9780805346138

ISBN10:
0805346139
Media:
Paperback
Pub. Date:
1/1/2006
Publisher(s):
Prentice Hall
List Price: $78.40
More New and Used
from Private Sellers
Starting at $3.94

Rent Textbook

We're Sorry
Sold Out

Used Textbook

We're Sorry
Sold Out

eTextbook

We're Sorry
Not Available

New Textbook

We're Sorry
Sold Out

Summary

KEY BENEFIT: Many biochemistry lab instructors are now opting to either design their own experiments or select them from major educational journals.Biochemistry Laboratory: Modern Theory and Techniquesaddresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. Part I: Theory and Experimental Techniques,Introduction to the Biochemistry Laboratory, The Computer as a Tool in Biochemistry and Molecular Biology, General Laboratory Procedures, Centrifugation Techniques in Biochemistry, Purification and Identification of Biomolecules by Chromatography, Characterization of Proteins and Nucleic Acids by Electrophoresis, Spectroscopic Analysis of Biomolecules, Biomolecular Interactions: Ligand Binding and Enzyme Reactions, Molecular Biology I: Structures and Analysis of Nucleic Acids, Molecular Biology II: Recombinant DNA. Molecular Cloning, and Enzymology, Protein Production, Purification, and Characterization, Part II: Teaching the Biochemistry/Molecular Biology Lab, A Brief History, A Variety of Teaching Methods, Essential BMB Concepts and Skills for Student Learning, Experiments in Biochemistry and Molecular BiologyKEY MARKET: For all readers interested in laboratory experiments.

Table of Contents

Introduction to the Biochemistry Laboratory
1(34)
Safety in the Laboratory
2(3)
Safety First
2(1)
Material Safety Data Sheets
2(2)
Safe Practices in the Biochemistry Laboratory
4(1)
Keeping Records and Communicating Experimental Results
5(9)
The Laboratory Notebook
5(2)
Details of the Experimental Write-Up
7(2)
Communicating Results from Biochemistry Research
9(5)
Using Biochemical Reagents and Solutions
14(4)
Water Quality
14(1)
Cleaning Laboratory Glassware
15(1)
Solutions: Concentrations and Calculations
15(2)
Preparing and Storing Solutions
17(1)
Quantitative Transfer of Liquids
18(5)
Pipets and Pipetting
18(3)
Automatic Pipetting Devices
21(2)
Statistical Analysis of Experimental Data
23(12)
Defining Statistical Analysis
23(1)
The Mean, Sample Deviation, and Standard Deviation
24(4)
Spreadsheet Statistics
28(1)
Statistical Analysis in Practice
28(2)
Study Problems
30(2)
Further Reading
32(3)
The Computer as a Tool in Biochemistry and Molecular Biology
35(20)
Computers in Biochemistry and Molecular Biology
35(3)
Personal Computing in Biochemistry
36(1)
The Computer and the Internet
37(1)
The World Wide Web
38(4)
Web Sites for Biochemistry and Molecular Biology
38(4)
Applications of the Web
42(13)
The Biochemical Literature
42(4)
Web Directories, Tools, and Databases
46(4)
Study Problems
50(2)
Further Reading
52(1)
Glossary for the Internet
53(2)
General Laboratory Procedures
55(46)
pH, Buffers, Electrodes, and Biosensors
55(14)
Measurement of pH
56(1)
Using the pH Electrode
56(2)
Biochemical Buffers
58(2)
Selection of a Biochemical Buffer
60(5)
Buffer Dilutions
65(1)
The Oxygen Electrode
66(2)
Biosensors
68(1)
Measurement of Protein Solutions
69(5)
The Biuret and Lowry Assays
70(2)
The Bradford Assay
72(1)
The BCA Assay
72(1)
The Spectrophotometric Assay
73(1)
Measurement of Nucleic Acid Solutions
74(2)
The Spectrophotometric Assay
74(1)
Other Assays for Nucleic Acids
74(2)
Techniques for Sample Preparation
76(7)
Dialysis
76(1)
Ultrafiltration
77(4)
Lyophilization and Centrifugal Vacuum Concentration
81(2)
Radioisotopes in Biochemistry
83(18)
Origin and Properties of Radioactivity
84(4)
Detection and Measurement of Radioactivity
88(7)
Radioisotopes and Safety
95(1)
Study Problems
95(2)
Further Reading
97(4)
Centrifugation Techniques in Biochemistry
101(22)
Basic Principles of Centrifugation
101(4)
Instrumentation for Centrifugation
105(8)
Low-Speed Centrifuges
106(1)
High-Speed Centrifuges
107(4)
Ultracentrifuges
111(2)
Applications of Centrifugation
113(10)
Preparative Techniques
113(1)
Analytical Measurements
114(4)
Care of Centrifuges and Rotors
118(2)
Study Problems
120(1)
Further Reading
121(2)
Purification and Identification of Biomolecules by Chromatography
123(52)
Introduction to Chromatography
124(2)
Partition versus Adsorption Chromatography
125(1)
Paper and Thin-Layer Chromatography (Planar Chromatography)
126(4)
Preparation of the Sorbent
126(1)
Solvent Development
127(1)
Detection and Measurement of Components
128(1)
Applications of Planar Chromatography
129(1)
Column Chromatography
130(4)
Operation of a Chromatographic Column
131(1)
Packing the Column
131(1)
Loading the Column
132(1)
Eluting the Column
132(1)
Collecting the Eluent
133(1)
Detection of Eluting Components
133(1)
Ion-Exchange Chromatography
134(6)
Ion-Exchange Resins
135(1)
Selection of the Ion Exchanger
136(2)
Choice of Buffer
138(1)
Preparation of the Ion Exchanger
138(1)
Using the Ion-Exchange Resin
138(1)
Storage of Resins
139(1)
Gel Exclusion Chromatography
140(9)
Theory of Gel Filtration
140(1)
Physical Characterization of Gel Chromatography
141(1)
Chemical Properties of Gels
142(1)
Selecting a Gel
142(2)
Gel Preparation and Storage
144(1)
Operation of a Gel Column
144(2)
Applications of Gel Exclusion Chromatography
146(3)
High-Performance Liquid Chromatography (HPLC)
149(14)
Instrumentation
150(4)
Stationary Phases in HPLC
154(2)
Chiral Chromatography
156(2)
The Mobile Phase
158(1)
Solvents for HPLC Operations
158(1)
Gradient Elution in HPLC
158(2)
Sample Preparation and Selection of HPLC Operating Conditions
160(1)
FPLC--A Modification of HPLC
161(1)
Perfusion Chromatography
162(1)
Affinity Chromatography and Immunoadsorption
163(12)
Chromatographic Media
164(1)
The Immobilized Ligand
165(1)
Attachment of Ligand to Matrix
165(3)
Immunoadsorption
168(1)
Experimental Procedure for Affinity Chromatography
168(2)
Study Problems
170(1)
Further Reading
171(4)
Characterization of Proteins and Nucleic Acids by Electrophoresis
175(38)
Theory of Electrophoresis
176(1)
Introduction
176(1)
Theory and Practice
176(1)
Methods of Electrophoresis
177(23)
Polyacrylamide Gel Electrophoresis (PAGE)
177(4)
Discontinuous Gel Electrophoresis
181(3)
Sodium Dodecyl Sulfate--Polyacrylamide Gel Electrophoresis (SDS-Page)
184(2)
Nucleic Acid Sequencing Gels
186(1)
Agarose Gel Electrophoresis
187(4)
Pulsed Field Gel Electrophoresis (PFGE)
191(2)
Isoelectric Focusing of Proteins
193(2)
Two-Dimensional Electrophoresis (2-DE) of Proteins
195(2)
Capillary Electrophoresis (CE)
197(2)
Immunoelectrophoresis (IE)
199(1)
Practical Aspects of Electrophoresis
200(13)
Instrumentation
200(1)
Reagents
200(1)
Staining and Detecting Electrophoresis Bands
201(3)
Protein and Nucleic Acid Blotting
204(1)
The Western Blot
204(5)
Analysis of Electrophoresis Results
209(1)
Study Problems
209(2)
Further Reading
211(2)
Spectroscopic Analysis of Biomolecules
213(40)
Ultraviolet-Visible Absorption Spectrophotometry
214(18)
Wavelength and Energy
214(1)
Light Absorption
215(2)
Electronic Transitions in Biomolecules
217(2)
The Absorption Spectrum
219(1)
The Beer-Lambert Law
220(1)
Instrumentation
221(4)
Applications of UV-VIS Spectroscopy
225(7)
Fluorescence Spectrophotometry
232(7)
Principles
232(1)
Quantum Yield
233(2)
Instrumentation
235(1)
Applications of Fluorescence Spectroscopy
236(1)
Difficulties in Fluorescence Measurements
237(2)
Nuclear Magnetic Resonance Spectroscopy
239(4)
NMR Theory
239(1)
NMR in Biochemistry
239(1)
NMR and Protein Structures
240(3)
Mass Spectrometry
243(3)
Ionization and Analysis of Proteins
244(1)
MS Applications in Biochemistry
245(1)
X-Ray Crystallography
246(7)
Methodology of X-ray Crystallography
247(1)
Study Problems
248(1)
Further Reading
249(4)
Biomolecular Interactions: Ligand Binding and Enzyme Reactions
253(32)
Ligand-Macromolecule Interactions (Molecular Recognition)
253(12)
Properties of Noncovalent Binding Interactions
254(2)
Quantitative Characterization of Ligand Binding
256(3)
Scatchard's Equation
259(1)
Cooperative Binding of Ligands
259(1)
Experimental Measurement of Ligand-Binding Interactions
260(1)
The Bradford Protein Assay as an Example of Ligand Binding
261(3)
Computer Software for Analysis of LM Binding
264(1)
Biological Catalysis (Enzymes)
265(20)
Classes of Enzymes
265(2)
Kinetic Properties of Enzymes
267(1)
Significance of Kinetic Constants
268(2)
Inhibition of Enzyme Activity
270(2)
Units of Enzyme Activity
272(1)
Specific Activity
273(1)
Design of an Enzyme Assay
273(1)
Kinetic versus Fixed-Time Assay
274(1)
Applications of an Enzyme Assay
275(2)
Computer Software for Analysis of Enzyme Kinetic Data
277(1)
Study Problems
278(2)
Further Reading
280(5)
Molecular Biology I: Structures and Analysis of Nucleic Acids
285(24)
Introduction to the Nucleic Acids
285(8)
Chemical Components of DNA and RNA
285(3)
DNA Structure and Function
288(3)
RNA Structure and Function
291(2)
Laboratory Methods for Investigation of DNA and RNA
293(16)
Isolation of Chromosomal DNA
293(2)
Isolation of Plasmid DNA
295(2)
Characterization of DNA
297(2)
Ethidium Bromide Binding and Fluorescence
299(1)
Agarose Gel Electrophoresis
300(1)
Sequencing DNA Molecules
301(2)
Isolation and Characterization of RNA
303(1)
Study Problems
304(1)
Further Reading
305(4)
Molecular Biology II: Recombinant DNA, Molecular Cloning, and Enzymology
309(20)
Recombinant DNA Biotechnology
309(8)
Molecular Cloning
310(2)
Steps for Preparing Recombinant DNA
312(2)
Cloning Vectors
314(3)
Important Enzymes in Molecular Biology and Biotechnology
317(8)
The Restriction Endonucleases
317(1)
Applications of Restriction Enzymes
318(2)
Practical Aspects of Restriction Enzyme Use
320(1)
The Polymerase Chain Reaction
321(4)
Nucleic Acid Blotting
325(4)
Study Problems
325(1)
Further Reading
326(3)
Protein Production, Purification, and Characterization
329(42)
Procedures for the Purification of Proteins
329(10)
Composition of Proteins
330(1)
Amount of Protein versus Purity of Protein versus Expense
331(1)
Basic Steps in Protein Purification
332(1)
Preparation of the Crude Extract
333(1)
Stabilization of Proteins in a Crude Extract
334(3)
Separation of Proteins Based on Solubility Differences
337(1)
Selective Techniques in Protein Purification
338(1)
Production of Proteins by Expression of Foreign Genes
339(5)
Gene Expression in Prokaryotic Organisms
340(3)
Gene Expression in Eukaryotic Cells
343(1)
Protein Characterization
344(1)
Determination of Primary Structure
345(8)
Amino Acid Composition
345(5)
Sequencing DNA Instead of the Protein
350(1)
Study Problems
350(1)
Further Reading
351(2)
Appendices
Appendix I List of Software Programs and Web Sites Useful for Each Chapter
353(2)
Appendix II Properties of Common Acids and Bases
355(1)
Appendix III Properties of Common Buffer Compounds
356(1)
Appendix IV pKa and pH1 Values of Amino Acids
357(1)
Appendix V Molecular Weight of Some Common Proteins
358(1)
Appendix VI Common Abbreviations Used in This Text
359(2)
Appendix VII Units of Measurement
361(2)
Appendix VIII Table of the Elements
363(2)
Appendix IX Answers to Selected Study Problems
365(6)
Index 371


Please wait while the item is added to your cart...