Cytometry, Part B: Wilson; Matsudaira; Darzynkiewicz; Robinson; Crissman: 9780122030543: Book: Science: eCampus.com

Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.

Key Features
* Contains 56 extensive chapters authored by world authorities on cytometry
* Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases
* Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine
* Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles
New York Medical College, Hawthorne. Presents a detailed discussion of all areas associated with the field of cytometry, including current, key methodologies and protocols essential to basic and clinical research. Includes new sections on technology principles. Plastic-comb binding, hardcover also available.

Contents of Volume 63

xii

Contributors

Preface to the Third Edition

xix

Preface to the Second Edition

xxiii

Preface to the First Edition

xxvii

PART VII Cytogenetics and Molecular Genetics

Sorting of Plant Chromosomes

Jaroslav Dolezel

Martin A. Lysak

Marie Kubalakova

Hana Simkova

Jiri Macas

Sergio Lucretti

Introduction

(2)

Application

(1)

Materials

(6)

Procedures

(6)

Critical Aspects of the Procedures

(3)

Instruments

(1)

Results

(5)

Conclusions and Perspectives

(7)

References

(5)

Quantitative DNA Fiber Mapping

Heinz-Ulli G. Weier

Introduction

(2)

Materials

(3)

Protocols

(8)

Critical Aspects of the Procedure

(1)

Results and Discussion

(8)

References

(3)

Primed in Situ Labeling

Johnny Hindkjaer

Lars Bolund

Steen Kolvraa

Introduction

(1)

Applications

(1)

Materials

(2)

Protocols

(8)

Critical Aspects of the Procedures

(4)

References

(3)

Measurements of Telomere Length on Individual Chromosomes by Image Cytometry

Steven S. S. Poon

Peter M. Lansdorp

Introduction

(2)

Background

(5)

Methods

(11)

Results

(4)

Discussion

(6)

References

(4)

Detection of Chromosome Translocation Products in Single Interphase Cell Nuclei

Jingly Fung

Santiago Munne

Heinz-Ulli G. Weier

Introduction

(1)

Materials

(3)

Protocols

102

(6)

Results and Discussion

108

(3)

Critical Aspects of the Procedure

111

(1)

Applications

112

(6)

References

113

(5)

PART VIII Cell Function and Differentiation

Analysis of Mitochondria by Flow Cytometry

Martin Poot

Robert H. Pierce

Introduction

118

(3)

Materials and Methods

121

(4)

Critical Aspects

125

(4)

References

126

(3)

Analysis of RNA Synthesis by Cytometry

Peter Østrup Jensen

Jacob Larsen

Jorgen K. Larsen

Introduction

129

(1)

Background

130

(1)

Methods for Analysis of 5'-Bromouridine Incorporation and DNA Content

131

(3)

Results of Labeling RNA with 5'-Bromouridine

134

(2)

Applications

136

(3)

References

137

(2)

Flow Cytometry of Erythropoiesis in Culture: Bivariate Profiles of Fetal and Adult Hemoglobin

Ralph M. Bohmer

Introduction

139

(2)

Details of the Method

141

(2)

Sample Experiments

143

(6)

Problems and Limitations

149

(4)

References

151

(2)

Flow Cytometric Analysis of Human Hemopoietic Progenitor Differentiation by Assessing Cell Division Rate and Phenotypic Profile

Luca Pierelli

Giovanni Scambia

Andrea Fattorossi

Introduction

153

(2)

Background

155

(1)

Critical Aspects of Methodology

156

(1)

Functionally Distinct Circulating Hemopoietic Progenitor Subsets Can Be Assessed during Cytokine-Driven Differentiation

157

(10)

Concluding Remarks

167

(6)

References

169

(4)

PART IX Experimental Oncology

Cytometry of Antitumor Drug--Intracellular Target Interactions

Paul J. Smith

Marie Wiltshire

Introduction

173

(1)

General Classification of Cytotoxic Anticancer Agents

174

(4)

Establishment of Quality Control Parameters

178

(5)

Drug-DNA Interactions

183

(5)

Conclusions

188

(5)

References

190

(3)

Monitoring of Cellular Resistance to Cancer Chemotherapy: Drug Retention and Efflux

Awtar Krishan

Introduction and Background

193

(3)

Applications

196

(1)

Cell Lines, Efflux, Multiple Drug Resistance Drugs, and Blockers

196

(1)

Staining Protocols

197

(1)

Critical Aspects

198

(1)

Controls, Standards, and Instruments

199

(2)

Results and Conclusions

201

(10)

References

206

(5)

Resistance of Tumor Cells to Chemo- and Radiotherapy Modulated by the Three-Dimensional Architecture of Solid Tumors and Spheroids

Ralph E. Durand

Peggy L. Olive

Introduction and Historical Perspective

211

(2)

Background

213

(2)

Methods

215

(2)

Results and Discussion

217

(10)

Conclusions and Future Directions

227

(8)

References

228

(7)

Analysis of DNA Damage in Individual Cells

Peggy L. Olive

Ralph E. Durand

Judit P. Banath

Peter J. Johnston

Introduction

235

(1)

The Development of the Single Cell Gel Electrophoresis/Comet Assay

236

(2)

Comet Preparation and Analysis

238

(4)

Types of Damage Detected by the Comet Assay

242

(2)

Using DNA Damage to Predict Cell Survival in Complex Systems

244

(2)

Future Directions

246

(5)

References

247

(4)

Cytometric Methods to Analyze Ionizing-Radiation Effects

William D. Wright

Isabelle Lagroye

Peng Zhang

Robert S. Malyapa

Joseph L. Roti Roti

Introduction

251

(1)

Applications

252

(1)

Methods for Measuring DNA Damage on a Cell-by-Cell Basis

253

(14)

Summary

267

(2)

References

267

(2)

Cytometric Methods to Analyze Thermal Effects

Robert P. VanderWaal

Ryuji Higashikubo

Mai Xu

Douglas R. Spitz

William D. Wright

Joseph L. Roti Roti

Introduction

269

(1)

Application

270

(1)

Methods to Measure Nuclear and Nuclear Matrix Protein Content

271

(5)

Identification of Altered DNA Replication Patterns Following Heat Shock

276

(2)

Nuclear Localization of hsp70

278

(2)

Proxidant Measurement

280

(1)

Results

281

(8)

References

285

(4)

PART X Clinical Oncology

Multiparameter Data Acquisition and Analysis of Leukocytes by Flow Cytometry

Carleton C. Stewart

Sigrid J. Stewart

Introduction

289

(1)

Correlated List Mode Data

290

(3)

Verification of Instrument Performance

293

(2)

Optical Filtration and Spectral Compensation

295

(13)

Multiparameter Data Analysis

308

(3)

Summary

311

(2)

References

311

(2)

Immunophenotyping of Hematological Malignancies by Laser Scanning Cytometry

Richard J. Clatch

Immunophenotyping of Leukemia and Lymphoma: General Considerations

313

(3)

Description of Laser Scanning Cytometry

316

(1)

Immunophenotyping by Laser Scanning Cytometry

316

(11)

Extensions of the Method

327

(13)

Conclusions

340

(4)

References

340

(4)

Immunophenotyping of Acute Leukemia: Utility of CD45 for Blast Cell Identification

J-P. Vial

F. Lacombe

Introduction

344

(1)

Background

344

(1)

Methods

345

(3)

Results and Comparison with Other Methods

348

(4)

Critical Aspects of the Methodology

352

(1)

Pitfalls and Misinterpretation of the Data

353

(2)

Future Directions

355

(4)

References

357

(2)

Cell Proliferation Markers in Human Solid Tumors: Assessing Their Impact in Clinical Oncology

Maria Grazia Daidone

Aurora Costa

Rosella Silvestrini

Introduction

359

(2)

Proliferation Markers

361

(4)

Biological Studies

365

(1)

Clinical Studies

366

(11)

Conclusions on First Generation Translational Studies with Proliferation Markers

377

(8)

References

378

(7)

Detection of Minimal Residual Disease

Andrzej Deptala

Sharon P. Mayer

Introduction

385

(2)

Tissue Sources to Detect Minimal Residual Disease

387

(1)

Methods to Detect Minimal Residual Disease

388

(26)

Technical Problems

414

(2)

Concluding Remarks

416

(6)

References

416

(6)

Analysis of Human Tumors by Laser Scanning Cytometry

Wojciech Gorczyca

Andrzej Deptala

Elzbieta Bedner

Xun Li

Myron R. Melamed

Zbigniew Darzynkiewicz

Introduction

422

(1)

Analysis of Cellular DNA Content by Laser Scanning Cytometry

423

(2)

Analysis of Apoptosis by Laser Scanning Cytometry

425

(5)

Analysis of Proliferation Associated Antigens by Laser Scanning Cytometry

430

(5)

Analysis of Estrogen Receptors by Laser Scanning Cytometry

435

(2)

Analysis of Transcription Factors by Laser Scanning Cytometry

437

(1)

Measurement of Nucleoli Using Laser Scanning Cytometry Fluorescence in Situ Hybridization Protocol

438

(8)

References

441

(5)

Laser Cytometry of Human Tissues and Tumors: Proliferation and Therapeutic Applications

David A. Rew

Introduction

446

(1)

Ploidy and Proliferation in Surgical Oncology

447

(1)

Cytometric Studies of Proliferation

448

(1)

The Halogenated Pyrimidines in Cell Proliferation Research

449

(3)

Clinical Studies of Cell Production Rates with Thymidine Analogs

452

(22)

Further Applications of Cytometry in Clinical Oncology

474

(4)

Conclusions

478

(10)

References

478

(10)

Prediction and Precise Diagnosis of Diseases by Data Pattern Analysis in Multiparameter Flow Cytometry: Melanoma, Juvenile Asthma, and Human Immunodeficiency Virus Infection

Gunter Valet

Hanna Kahle

Friedrich Otto

Edeltraut Brautigam

Luc Kestens

Introduction

488

(1)

Material and Methods

489

(4)

Results

493

(13)

Discussion

506

(5)

References

507

(4)

PART XI Microorganisms and Infectious Diseases

Flow Cytometric Analysis of Microorganisms

S. A. Sincock

J. Paul Robinson

Introduction

511

(3)

Experimental Approaches

514

(12)

Applications in Medical and Food Microbiology

526

(5)

Conclusion

531

(8)

References

532

(7)

Staining and Measurement of DNA in Bacteria

Harald B. Steen

Introduction

539

(1)

Basic Considerations

540

(3)

Experimental Methods

543

(5)

Standards and Controls

548

(5)

References

550

(3)

Flow Cytometric Monitoring of Bacterial Susceptibility to Antibiotics

Mette Walberg

Harald B. Steen

Introduction

553

(1)

Fluorescent Dyes

554

(1)

Uptake of Fluorescent Dyes

555

(3)

Effects of Antibiotics

558

(1)

Assessment of Drug Effects

558

(5)

Applications of Flow Cytometry to Medical Microbiology

563

(4)

References

563

(4)

Flow Cytometry for Evaluation and Investigation of Human Immunodeficiency Virus Infection

Thomas W. Mc Closkey

Introduction

567

(1)

Application of Flow Cytometry to Monitor HIV Infection

568

(14)

Application of Flow Cytometry to Investigate HIV Disease

582

(2)

Conclusions

584

(9)

References

584

(9)

Index

593

(16)

Volumes in Series

609


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