PCR/RT- PCR in situ: Light and Electron Microscopy

This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.
-- introduces new techniques to detect foreign genes within cells
-- Presents detailed protocols in step-by-step format
-- Provides a broad range of applications in the diagnosis of infectious diseases
-- Provides preparation and storage instructions for materials, reagents, and solutions