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IMMUNE AND RECEPTOR ASSAYS IN THEORY AND PRACTICE,9780849300615
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IMMUNE AND RECEPTOR ASSAYS IN THEORY AND PRACTICE


Edition: 1st
Author(s): Englebienne, Patrick
ISBN10:  0849300614
ISBN13:  9780849300615
Format:  Hardcover
Pub. Date:  12/28/1999
Publisher(s): CRC

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SummaryTable of Contents
Free Univ. of Brussels, Belgium. Presents the fundamentals of techniques of these assays and introduces examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. For researcher and development scientists and others in contact with immuno- or receptor-assay technology.
Introduction v
Receptors at Work
1(22)
Bibliographical References and Further Reading
20(3)
Receptors and Antibodies
23(36)
Origin and relatedness
23(8)
Structure, biosynthesis, and interactions
31(17)
The steroid and thyroid hormone receptor superfamily
32(3)
The circulating steroid-binding proteins
35(6)
The immunoglobulin-like superfamily
41(7)
Biological, biosynthetic, and synthetic alternatives
48(8)
Polyspecific antibodies
49(1)
Oligonucleotides
50(3)
Imprinted polymers
53(3)
Bibliographical References and Further Reading
56(3)
Receptor and Antibody Production
59(82)
Cell culture and receptor production
59(15)
Antigens, haptens, and immunogenicity
74(16)
Production of monoclonal and polyclonal antibodies
90(15)
Extraction, purification, and stabilization of receptors and antibodies
105(30)
Bibliographical References and Further Reading
135(6)
Molecular Basis of Ligand-Receptor Interaction
141(40)
The molecular recognition process
141(4)
Kinetics of ligand-receptor interactions
145(13)
Physical aspects of ligand-receptor interactions
158(13)
Factors influencing ligand-receptor interactions
171(7)
Bibliographical References and Further Reading
178(3)
Immuno- and Receptor-Assay Design
181(46)
Competitive versus noncompetitive assay
181(8)
Homogeneous versus heterogeneous---liquid versus solid phase assay
189(11)
Agglutination and preceipitation assays
200(12)
Assays using labeled reagents
212(10)
Market or technology driven assay design
222(1)
Bibliographical References and Further Reading
223(4)
Analytical Signal: Generation and Detection
227(64)
Labels and their measurement
227(26)
Techniques of labeling
253(13)
Tracer purification and stabilization
266(8)
Biosensors
274(3)
Instrumentation
277(8)
Bibliographical References and Further Reading
285(6)
The Sample
291(34)
Insoluble versus soluble analyte
291(9)
Matrix effects
300(11)
Standardization
311(11)
Bibliographical References and Further Reading
322(3)
Assay Validation
325(30)
The reference method
325(13)
Specificity and sensitivity
338(6)
Interferences
344(8)
Bibliographical References and Further Reading
352(3)
Data Analysis, Processing, and Presentation
355(26)
The dose-response curve
355(7)
Binding data
362(10)
Reagent stability follow-up
372(5)
Assay validation
377(3)
Bibliographical References and Further Reading
380(1)
Index 381

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