| Foreword |
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v | |
| Preface |
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vii | |
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Molecules for Gene Therapy |
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Recent Progress in Non-viral Gene Delivery |
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3 | (8) |
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Physical Chemistry of DNA-Carrier Complexes |
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11 | (8) |
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19 | (16) |
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35 | (16) |
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Development of a Supramolecular Nanocarrier for Gene Delivery Based on Cationic Block Copolymers |
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51 | (12) |
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63 | (12) |
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Dendrimers as DNA Carriers |
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75 | (12) |
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Use of Synthetic Peptides for Non-viral Gene Delivery |
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87 | (16) |
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An Oligonucleotide Carrier Based on β-1,3-Glucans |
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103 | (15) |
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Biological and Chemical Hybrid Vectors |
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118 | (17) |
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Pharmacokinetics of Gene Delivery in Cells |
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135 | (20) |
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Active DNA Release from Complexes |
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155 | (10) |
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Controlled Release of DNA Using Thermoresponsive Polymers |
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165 | (11) |
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Active Transport of Exogenous Genes into the Nucleus |
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176 | (11) |
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Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation |
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187 | (11) |
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Functional Nucleotide Sequences Capable of Promoting Non-viral Genetic Transfer |
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198 | (13) |
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Shielding of Cationic Charge of the DNA Complex to Avoid Nonspecific Interactions for In Vivo Gene Delivery |
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211 | (15) |
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In Vivo Gene Transfer by Ligand-Modified Gene Carriers |
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226 | (11) |
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Optimizing Polyplexes into Synthetic Viruses for Tumor-Targeted Gene Therapy |
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237 | (9) |
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Gene Transfer and Target Diseases |
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246 | (15) |
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Clinical Trials Using Non-viral Gene Delivery Systems |
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261 | (32) |
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Current Protocols of Gene Delivery |
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Evaluation of Size and Zeta Potential of DNA/Carrier Complexes |
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293 | (7) |
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Observation of DNA/Carrier Complexes Under Fluorescence Microscopy |
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300 | (7) |
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Synthesis of Chemically Modified Chitosan and a Study of Its Gene Transfection Efficiency |
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307 | (8) |
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Newly Designed DNA Fragments for Gene Correction |
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315 | (8) |
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Evaluation of Gene Expression In Vivo After Intravenous and Intraportal Administration of Lipoplexes |
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323 | (8) |
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Evaluation by Southern Blot Hybridization of DNA Administered with a Gene Carrier to Organs |
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331 | (8) |
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Evaluation of the Immune Response After Administration of Plasmid DNA-Non-viral Vector Complexes |
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339 | (9) |
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348 | (15) |
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Design of Genes Based on Current RNA Technology |
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Design of Intracellularly Active Ribozymes and siRNAs |
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363 | (35) |
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RNAi-Based Inhibition Specific for Mutant Alleles in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discrimination of Mutant and Wild-Type Alleles by siRNA |
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398 | (7) |
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In Vivo RNA Interference: Another Tool in the Box? |
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405 | (18) |
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Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription |
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423 | (18) |
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Intracellular Delivery of Nucleic Acids: Differences Between Transfection and siFection Reflect Differences Between DNA and RNA, and Between Oligodeoxynucleotides and Oligonucleotides |
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441 | (15) |
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In Vivo Antitumor Activity of a New Cationic Liposome siRNA Complex |
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456 | (9) |
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Enhancing RNAi with Synthetic RNA Duplexes |
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465 | (12) |
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| Subject Index |
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477 | |
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