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Aiming to foster future collaborations between researchers in algorithms, bioinformatics, and molecular biology, this book serves as an up-to-date survey of the most important recent developments and computational challenges in various application areas of next-generation sequencing technologies. Offering helpful insight from renowned experts, the book covers topics such as NGS error correction, road mapping, variant detection and genotyping, characterization of structural variants with NGS, genome-assisted transcriptome reconstruction, small RNA analysis, and much more.