Ebola and Marburg Viruses : A View of Infection Using Electron Microscopy

by Ryabchikova, Elena; Price, Barbara B. S.

• ISBN13: 9781574771312
• ISBN10: 1574771310
• Format: Hardcover
• Copyright: 2002-08-01
• Publisher: Not Applicable

Summary

Consolidates recent literature with the insight developed in over thirty years in electron microscopy and 15 years in virology to tell the story of filoviruses and how they invade and conquer their hosts. The book describes the dynamic properties of these viruses and follows the stages of filoviral infection from the individual cell to the whole organism and reconstructs the sequential events that occur in filoviral infections.

Author Biography

Elena Ivanovna Ryabchikova, Ph.D., is the Head of Laboratory of Ultrastructural Investigations and Pathomorphology at SRC VB Vector in Novosibirsk, Region of Siberia Russia Barbara Breidenbach Saunders-Price, Ph.D., is a Senior Research Scientist at Battelle Science and Technology International in Aberdeen, Maryland, USA

Table of Contents

Frontispiece	p. ii
Dedication	p. v
Acknowledgements	p. vii
Figures and Tables	p. xiii
Preface	p. xxi
Introduction	p. 1
Viruses and Virology	p. 1
Filoviruses	p. 4
Filoviruses: A Review of Their History	p. 5
Filoviruses: Taxonomy	p. 8
Materials and Methods	p. 11
Viruses	p. 11
Animals and Cell Cultures	p. 12
Electron Microscopy Sample Preparation	p. 15
Summary	p. 18
Morphology of Filoviruses	p. 19
Filoviral Shapes	p. 19
General Structure of Filoviruses	p. 23
Structure and Function of Filoviral Proteins	p. 24
Infection: Penetration	p. 26
Infection: Morphology of Viral Replication	p. 32
Infection: Viral Budding	p. 42
Conclusion	p. 53
Filoviral Infections	p. 57
Filoviral Reservoirs	p. 57
Transmission Between Humans	p. 60
Experimental Studies of Filvorial Infection	p. 61
Transmission Mode and Disease	p. 61
Primary Target Cells	p. 63
Lymph Nodes	p. 63
Injection Site Localization	p. 64
Macrophage Cells and Susceptibility	p. 66
Aerosol Infection	p. 70
Secondary Target Cells	p. 71
Other Target Cells	p. 77
Conclusion	p. 80
Microscopic Pathology over the Course of Filoviral Infection	p. 85
Experimental Design	p. 86
Filoviral Reproduction	p. 88
Blood System	p. 88
Liver	p. 89
Spleen	p. 97
Kidneys	p. 97
Lungs	p. 97
Adrenals	p. 100
Heart	p. 100
Digestive Tract	p. 100
Summary of Target Cells and Target Organs	p. 101
Blood Disorders in Filoviral Infections	p. 105
Clinical Manifestations	p. 105
Morphologic Observations	p. 107
Time Course of Pathomorphological Changes in the Blood System	p. 108
Species-Related Differences in EBOV-Infected Monkeys	p. 118
Possible Mechanisms	p. 121
Blood Cell Counts	p. 125
Conclusion	p. 128
Time Course of the Pathological Changes in Filovirus-Infected Animals	p. 131
Liver	p. 132
Pathological Changes	p. 132
Inflammation	p. 137
Spleen	p. 140
Kidneys	p. 145
Lungs	p. 151
Adrenals	p. 153
Heart	p. 156
Digestive Tract	p. 157
Summary	p. 158
Immunopathology	p. 159
Filoviral Reproduction In Lymphatic Tissue	p. 160
Pathological Changes	p. 161
Formation of Lymphocytes	p. 163
Lymphoid Depletion	p. 163
Damage of Macrophages, Stromal, and Dendritic Cells	p. 168
Other Pathological Changes	p. 170
Lack of Inflammation	p. 173
Immune Responses in Human Filoviral Infections	p. 173
Possible Mechanisms of Immunity Impairment and Directions of Future Research	p. 174
Conclusions	p. 177
Filoviral Infection	p. 177
Disease and Pathology	p. 179
Inflammatory Response	p. 180
Animal Models for Filoviral Studies	p. 181
What Directly Causes Rapid Death in Filoviral Infections?	p. 182
Crucial Role of the Macrophages	p. 183
Systemic Aspects of Fatal EBOV and MARV Infections	p. 184
References	p. 187
Index	p. 205
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