9781420093261

Experimental Surgical Models in the Laboratory Rat

by ;
  • ISBN13:

    9781420093261

  • ISBN10:

    1420093266

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2009-05-19
  • Publisher: CRC Press

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Summary

Rosario National Univ., Argentina. Describes basics of surgical techniques used at the Rosario National University, the necessary materials, precautions to consider, methodology to apply, and possible results to be obtained in similar experiments. Includes experimental models and describes pre- and post-surgical procedures. For researchers. DNLM: Disease Models, Animal.

Table of Contents

Prefacep. xi
Acknowledgmentsp. xiii
The Editorsp. xv
Contributorsp. xvii
Abbreviationsp. xix
Introduction
Bioethics and Animal Carep. 3
Control of Stress and Distressp. 9
Management of Pain in Laboratory Animalsp. 17
Anesthesia and Analgesiap. 21
Euthanasiap. 31
Antibioticsp. 33
General Procedures
Operating Theatrep. 37
Rat Identificationp. 41
Antisepsis, Sterilization, and Asepsisp. 43
Suturep. 47
Substances Administrationp. 55
Samplesp. 63
Catheterization and Cannulation
Catheterization of the Femoral Artery and Veinp. 73
Cardiac Catheterizationp. 77
Cannulation of the Thoracic Ductp. 83
Tracheostomyp. 87
Gastrointestinal Tract
In Situ Isolation of the Stomachp. 91
In Situ Isolation of the Intestinal Loopp. 95
Intestinal Everted Sacsp. 99
In Situ Perfusion of the Small Intestinep. 103
Pancreas
Experimental Models for the Study of Diabetesp. 109
Islets of Langerhans Isolationp. 115
Incubation of Pancreatic Tissue Slicesp. 117
In Situ Perfusion of the Pancreasp. 121
Experimental Pancreatitisp. 125
Liver
Hepatic Circulation Impairmentp. 135
Extrahepatic Cholestasis Modelp. 139
Ablation of Endocrine Glands
Adrenalectomyp. 145
Ovariectomyp. 149
Thyroparathyroidectomyp. 153
Parathyroid Glandsp. 157
Kidneys
Measurement Techniques of Renal Parametersp. 161
Kidney Isolation and Perfusionp. 169
Acute Renal Failure Modelsp. 177
Renal Blood Flow Measurementp. 183
Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortexp. 189
Remnant Kidney Modelp. 195
Circulatory System
Experimental Myocardial Infarctionp. 201
Experimental Arteriosclerosisp. 205
Isolated Heart: Langendorff Techniquep. 209
Additional Techniques
Polyclonal Antibodies Preparationp. 217
Histological Proceduresp. 221
Bones and Bone Tissuep. 229
Miscellaneous
Equipmentp. 235
Solution Preparationp. 241
Normal Values in IIm/Fm Rat Subline "M"p. 243
Indexp. 247
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