Experimental Surgical Models in the Laboratory Rat

by Rigalli; Alfredo

• ISBN13: 9781420093261
• ISBN10: 1420093266
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-05-19
• Publisher: CRC Press

Summary

Rosario National Univ., Argentina. Describes basics of surgical techniques used at the Rosario National University, the necessary materials, precautions to consider, methodology to apply, and possible results to be obtained in similar experiments. Includes experimental models and describes pre- and post-surgical procedures. For researchers. DNLM: Disease Models, Animal.

Table of Contents

Preface	p. xi
Acknowledgments	p. xiii
The Editors	p. xv
Contributors	p. xvii
Abbreviations	p. xix
Introduction
Bioethics and Animal Care	p. 3
Control of Stress and Distress	p. 9
Management of Pain in Laboratory Animals	p. 17
Anesthesia and Analgesia	p. 21
Euthanasia	p. 31
Antibiotics	p. 33
General Procedures
Operating Theatre	p. 37
Rat Identification	p. 41
Antisepsis, Sterilization, and Asepsis	p. 43
Suture	p. 47
Substances Administration	p. 55
Samples	p. 63
Catheterization and Cannulation
Catheterization of the Femoral Artery and Vein	p. 73
Cardiac Catheterization	p. 77
Cannulation of the Thoracic Duct	p. 83
Tracheostomy	p. 87
Gastrointestinal Tract
In Situ Isolation of the Stomach	p. 91
In Situ Isolation of the Intestinal Loop	p. 95
Intestinal Everted Sacs	p. 99
In Situ Perfusion of the Small Intestine	p. 103
Pancreas
Experimental Models for the Study of Diabetes	p. 109
Islets of Langerhans Isolation	p. 115
Incubation of Pancreatic Tissue Slices	p. 117
In Situ Perfusion of the Pancreas	p. 121
Experimental Pancreatitis	p. 125
Liver
Hepatic Circulation Impairment	p. 135
Extrahepatic Cholestasis Model	p. 139
Ablation of Endocrine Glands
Adrenalectomy	p. 145
Ovariectomy	p. 149
Thyroparathyroidectomy	p. 153
Parathyroid Glands	p. 157
Kidneys
Measurement Techniques of Renal Parameters	p. 161
Kidney Isolation and Perfusion	p. 169
Acute Renal Failure Models	p. 177
Renal Blood Flow Measurement	p. 183
Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex	p. 189
Remnant Kidney Model	p. 195
Circulatory System
Experimental Myocardial Infarction	p. 201
Experimental Arteriosclerosis	p. 205
Isolated Heart: Langendorff Technique	p. 209
Additional Techniques
Polyclonal Antibodies Preparation	p. 217
Histological Procedures	p. 221
Bones and Bone Tissue	p. 229
Miscellaneous
Equipment	p. 235
Solution Preparation	p. 241
Normal Values in IIm/Fm Rat Subline "M"	p. 243
Index	p. 247
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