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Fundamental Laboratory Approaches for Biochemistry and Biotechnology, 2nd Edition,9780470087664
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Fundamental Laboratory Approaches for Biochemistry and Biotechnology, 2nd Edition

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This is the 2nd edition with a publication date of 5/1/2009.
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Ninfa/Ballou/Parsons is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Parsons focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.

Table of Contents

Basic Practices and Techniques in the Biochemistry Laboratory
Quantification of Protein Concentration
Gel Electrophoresis of Proteins
Overview of Protein Purification
Isolation and Characterization of the Enzyme Alkaline Phosphatase from Escheria Coli
Enzyme Kinetics
Enzymatic Methods of Analysis
Ligand Binding
Recombinant DNA Techniques
Polymerase Chain Reaction (PCR) Technology
Using the PC and the Internet for Biochemical Research
Table of Contents provided by Publisher. All Rights Reserved.

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