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9780470087664

Fundamental Laboratory Approaches for Biochemistry and Biotechnology

by ; ;
  • ISBN13:

    9780470087664

  • ISBN10:

    0470087668

  • Edition: 2nd
  • Format: Paperback
  • Copyright: 2009-05-26
  • Publisher: WILEY

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Summary

Ninfa/Ballou/Parsons is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Parsons focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.

Author Biography

Alexander J. Ninfa is Associate Professor in the Department of Biological Chemistry at the University of Michigan and is internationally recognized for his work on the biochemical mechanisms of signal transduction and transcriptional regulation in bacteria.

David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.

Table of Contents

Basic Practices and Techniques in the Biochemistry Laboratory
Spectrophotometry
Quantification of Protein Concentration
Chromatography
Gel Electrophoresis of Proteins
Overview of Protein Purification
Isolation and Characterization of the Enzyme Alkaline Phosphatase from Escheria Coli
Enzyme Kinetics
Enzymatic Methods of Analysis
Ligand Binding
Recombinant DNA Techniques
Polymerase Chain Reaction (PCR) Technology
Using the PC and the Internet for Biochemical Research
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

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