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Genetics Laboratory Investigations

by ;
Edition:
13th
ISBN13:

9780131742529

ISBN10:
0131742523
Format:
Paperback
Pub. Date:
6/13/2006
Publisher(s):
Benjamin Cummings
List Price: $103.20

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Summary

THEdefinitive genetics lab manual for over 50 years, this user-friendly volume stresses classical genetics, but includes some of the recent advances related to molecular and human genetics as well.Drosophilaand Maize Experiments in Genetics: Monohybrid Crosses; Dihybrid Crosses. Cell Reproduction: Mitosis. Meiosis in Animals: Oogenesis and Spermatogenesis. Meiosis in Angiosperms: Microsporogenesis and Megasporogenesis. Polytene Chromosomes fromDrosophilaSalivary Glands. Sex Chromosomes and Gene Transmission. The Sex Check: A Study of Sex Chromatin in Human Cells. Human Chromosomes. Linkage and Crossing Over. Genetics of Ascospore Color inSordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis. Open-Ended Experiments UsingDrosophila: Locating a Mutant Gene in Its Chromosome. Isolation of DNA. Restriction Endonuclease Digestion and Gel Electrophoresis of DNA. Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting. Transformation ofEscherichia coli.Gene Action: Synthesis of ... b-Galactosidase inEscherichia coli.Chromatographic Characterization ofDrosophilamelanogaster Mutants. Bacterial Mutagenesis. Gene Recombination in Phage. Polygenic Inheritance: Fingerprint Ridge Count. Population Genetics: The Hardy-Weinberg Principle; The Effects of Selection and Genetic Drift. Applied Human Genetics.For anyone interested in hands-on genetics work.

Table of Contents

Preface v
Drosophila and Maize Experiments in Genetics: Monohybrid and Dihybrid Crosses
1(18)
Principles of Probability
19(8)
The Chi-Square Test
27(14)
Cell Reproduction: Mitosis
41(8)
Meiosis in Animals: Oogenesis and Spermatogenesis
49(8)
Meiosis in Angiosperms: Microsporogenesis
57(14)
Polytene Chromosomes from Drosophila Salivary Glands
71(4)
Sex Chromosomes and Gene Transmission
75(10)
The Sex Check: A Study of Sex Chromatin in Human Cells
85(6)
Human Chromosomes
91(32)
Linkage and Crossing Over
123(20)
Genetics of Ascospore Color in Sordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis
143(8)
Open-Ended Experiments Using Drosophila: Locating a Mutant Gene in Its Chromosome
151(6)
The Genetic Material: Isolation of DNA
157(6)
Restriction Endonuclease Digestion and Gel Electrophoresis of DNA
163(16)
The Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting
179(16)
Transformation of Escherichia coli
195(10)
Gene Action: Synthesis of β-Galactosidase in Escherichia coli
205(6)
Chromatographic Characterization of Drosophila melanogaster Mutants
211(8)
Bacterial Mutagenesis
219(6)
Gene Recombination in Phage
225(6)
Polygenic Inheritance: Fingerprint Ridge Count
231(10)
Population Genetics: The Hardy-Weinberg Principle
241(12)
Population Genetics: The Effects of Selection and Genetic Drift
253(8)
Applied Human Genetics
261(20)
NCBI and Genomic Data Mining
281(18)
Supplemental Laboratory Topics
299(1)
Supplement 1 Plant Speciation by Interspecific Hybridization and Allotetraploidy
299(2)
Supplement 2 Chromosome Translocations in Rhoeo spathacea
301(1)
Supplement 3 Human Pedigree Analysis
302


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