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In the rapidly evolving field of genome sciences and technology, it is difficult for professional researchers to keep track of new knowledge and technology developments. This unique book attempts to remedy this situation by presenting the basic principles and technological foundations that support current and future advances in this exciting field. Also, the most state-of-the-art technologies which drive the development of this field are described in detail, with a special focus on genome engineering and synthetic genomics in the field of synthetic biology. This publication can be used and developed into teaching materials for graduate and advanced undergraduate students in both life sciences and engineering disciplines, and it is also suitable for researchers for reference, or non-experts who wish to have a basic overview of the subject.