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The Handbook of Plant Metabolomics,9783527327775
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The Handbook of Plant Metabolomics

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Metabolomics is the analysis of all metabolites such as metabolic intermediates, hormones and so on, in a given system such as a cell, an organ or a whole organism. It is complementary to the genomics and proteomic analysis of a system and an essential building block towards the understanding of a whole biological system. Being a relatively new approach, a number of techniques have been recently developed in order to experimentally analyse metabolites and many researchers are expanding into this field. Knowledge about the metabolic status of a system is critical for its comprehensive understanding and metobolomics is an important tool for systems biologists.

This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series, The Handbook of Plant Metabolomics presents an excellent overview of different approaches and techniques in the subject. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, this book is intended for molecular biologists, geneticists, biotechnologists, plant physiologists, gene technologists, protein chemists, biotechnological industry personel and the pharmaceutical industry.

With uniform chapter design with summaries and comprehensive glossary, the Kahl books are in addition a trusted brand for the plant science community. 

Author Biography

G?nter Kahl is Professor for Plant Molecular Biology at the Biocenter of Johann Wolfgang Goethe-University of Frankfurt am Main, Germany. After gaining his Ph.D. in plant biochemistry, he spent two postdoctoral years at Michigan State University, East Lansing (USA), joining Professor Joe Varner and Professor James Bonner at the California Institute of Technology, Pasadena, California. His main research interests focus on
- Gene Technology, in particular the genetic and physical mapping as well as isolation and characterization of plant defence genes and their promoters, and the use of in vitro modified defence genes for the improvement of plant crops via gene transfer.
- Genome Analysis, in particular the development of molecular marker , technologies and informative DNA markers for genomic fingerprinting, the establishment of genetic and physical maps, and the map-based cloning of agronomically important genes.
- Gene Expression Profiling of plant and animal tissues with expression microarrays and the patented high-throughput SuperSAGE technology. Development of expression markers and determination of expression phenotypes.

Due to the international nature of his work, Professor Kahl cooperates with a series of research institutions throughout Europe, Japan, the USA, Syria, India, Central and South America. Additionally, he is organizing a series of molecular marker courses in Austria, South America, Asia, Africa and the Middle East. He has served in expert missions for IAEA, FAO and UNESCO around the world.
Professor Kahl is the author of more than 250 scientific journal publications and several book publications including all titles of the successful book series `Molecular Plant Biology Handbook series?, and currently holds the CSO position at GenXPro GmbH, a company specializing in novel technologies in genomics and transcriptomics, located at the Frankfurt Innovation Centre for Biotechnology (FIZ), Frankfurt am Main, Germany.

Univ.-Prof. Dr. Wolfram Weckwerth
Professur f?r Molekulare Pflanzenphysiologie, Department f?r ?kophysiologie und Funktionelle Anatomie der Pflanzen, Universit?t Wien

- Entwicklung und Anwendung von genomweiten Metabolomics und Proteomics/Phosphoproteomics-Technologien in der Systembiologie; Hochdurchsatzprofilanalysen (HTP); Datenintegration; Kombination von HTP, multivariater Statistik, Mustererkennung in molekularen Daten und Stoffwechselmodellierung: "Synergetics"
- Entwicklung theoretischer Modelle, aus HTP-Daten biochemische Regulation abzuleiten *Pflanzen-Genotyp-Phenotyp-Interaktion, unter anderem Verwendung von Pflanzenmutanten zur funktionalen Untersuchung von phenotypischer Plastizit?t, Stress-, Wachstum-, Entwicklungs- und Stoffwechselphysiologie
- Genom-Annotation und Rekonstruktion von Stoffwechsel und Regulation in sequenzierten Modellorganismen, wie Arabidopsis thaliana (dicot), Oryza sativa (monocot), Chlamydomonas reinhardtii (einzellige Gr?nalge), Medicago truncatula (Leguminose) und andere, mithilfe von Metabolomics und Proteomics-Daten, Anwendung von Metabolomics und Proteomics auf nicht-sequenzierte Organismen wie Tomate, Kartoffel, Pflanzengemeinschaften, Biodiversit?t etc.
- HTP-Datenbanken, Datenmanagement und Data-Mining

Table of Contents


Table of Contents

List of Contributors


Ute G. Hofmann(Clinical Pharmacology, Stuttgart University, Germany): ?Good practice in metabolite profiling: A critical review.?

Ute Roessner (University of Melbourne, Australia): ?Plant metabolomis: An introduction.?

Metabolic Profiling

Mark Stitt (MPI Golm): ?Automation of enzymatic determination of metabolites and metabolite profiles?

Young Hae Choi (Leiden University, The Netherlands): ?NMR for plant metabolite profiling?

Aiko Barsch (Bruker Daltonik, Bremen, Germany): ?Metabolic profiling with LC-TOF-MS/MS combined with NMR?

Hans-Peter Deigner, Georg Debus (BIOCRATES Life Sciences, Austria): ?Quantitative metabolite analyses?

Peter Geigenberger (IPK, GABI BASIS: SysSEED): ?Flux patterning, structural and kinetic models?

Stefan Kempa (MPI Golm, Germany): ?TwoD GC/MS metabolomics?

J?rn Smeedsgard, Jens Nielsen (Technical University of Denmark, Lyngby, Denmark): ? Direct infusion electrospray mass spectrometry for mass profiling and species classification.?

Dirk Walther (MPI Golm, Germany ): ?Metabolite-enzyme-transcript correlations?

?Headspace trapping and GC-MS for the analysis of plant volatiles? (Author to be determined)

?Coupling of capillary electrophoresis and mass spectrometry? (Author to be determined)

J?rgen Kastler (metanomics GmbH, Berlin): ?High-throughput technologies for plant metabolite profiling?

Wolfgang Eisenreich (TU M?nchen, Germany): ?Isotopologue profiling and metabolic networks?

Uwe Sauer, Nicola Zamboni (ETH Z?rich, Switzerland): ?Metabolite flux analysis?

Thomas Gr?ger (Helmholtz Zentrum Munich, Germany): ?Two-dimensional gas chromatography (GCxGC) for metabolite profiling?

Michael Udvardee (Noble Foundation, Ardmore, USA): ?High-throughput screening for metabolite-protein interactions

Wolfram Weckwerth (Molekulare Systembiologie, Universit?t Wien, Austria):?Coregulation of transcripts, proteins and metabolites?

Chemical arrays (Author to be determined)

Applications of Metabolomics in Plant Biology

Alisdair Fernie (MPI Golm, Germany): ?Metabolomics and marker-assisted plant breeding?

Richard A. Dixon (The Nobel Foundation, Ardmore, USA):?Determining natural plant products: Phenylpropanoids, isoflavonoids, and saponins?

Jim Liao (UCLA, USA): ?DNA microarray analysis and metabolic engineering?

Jairus Bowne, Ute Roessner (Australian Centre for Plant Functional Genomics, University of Melbourne, Parkville, Victoria, Australia): ?Metabolic responses of plants to abiotic stress: Specific metabolites and metabolite levels in different species.?

Joachim Kopka, Lothar Willmitzer (MPI Golm, Germany): ?Metabolomics of stress acclimation?

Hans Weber (IPK Gatersleben, Germany): ?Overexpression of transgenes and plant metabolism?


Dirk Walther (MPI Golm, Germany): ?GMD: An advanced metabolite data base?

Heiko Neuweger (CeBiTec Bielefeld, Germany):?Analysis and integration of metabolome data: MeltDB?

Alexander Kaever (Uni G?ttingen, Germany): ?Metabolite clustering and visualization of mass spectrometry data using one-dimensional self-organizing maps?

Mark Stitt (MPI Golm, Germany): ?From metabolic data to pathway analysis
(MapMan, PageMan, MapScavenger, MapManWeb)?



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