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Written by an international team of experts from the Tara Oceans Marine Biology Imaging Platform (TAOMI), this is the first and only compendium on marine imaging technologies, and includes all known underwater as well as on-land techniques. TAOMI is imaging the largest collection of marine organisms in recent history, ranging from viruses to corals, and is duplicated on land to perform high throughput confocal analysis of plankton, X-ray tomography as well as cryo-electron microscopy. This unique platform combines underwater imaging with cytometry, stereomicroscopy, fluorescence microscopy and 3D microscopy ? all of which are covered in this practical book, along with remote sensing, MRI, and optical projection tomography. The definitive resource for every marine biologist who is planning to image marine species, whether underwater or on land.
Emmanuel G. Reynaud is heading the Tara Oceans Marine biology Imaging platform (TAOMI). Having obtained his PhD in Life Sciences from the University of Paris XI/Orsay, he spent most of his career working the European Molecular Biology Laboratory in Heidelberg Germany where he developed new optical method applied to biology including laser nanosurgery approaches. He was also involved in the development of the Light Sheet based Fluorescence Microscopy as a member of the Light Microscopy Group headed by Ernst H.K. Stelzer at EMBL. The TAOMI platform is on board the schooner Tara for a three years circumnavigation of the world ocean to collect and image the largest collection of marine species spanning from viruses to corals. This unique platform is duplicated on land to perform high throughput confocal analysis of plankton, X-ray tomography as well as cryo-electron microscopy. TAOMI combines underwater imaging with cytometry, stereomicroscopy, fluorescence microscopy and 3D microscopy.
Table of Contents
Preface Authors, Contributors Community Support Abbreviations
1 General Introduction: Imaging the largest biotope Emmanuel G. Reynaud, Michael Sieracki, Mark Benfield 2 Underwater imaging and remote sensing Mark Benfield 3 Flow cytometry applications Michael Sieracki, Peter Olson 4 FRRF Sasha Tozzi, Zbigniew Kolber 5 Microscopy applications Emmanuel G. Reynaud 6 MRI, X-ray tomography Renaud Boistel 7 Optical Projection Tomography Jim Swoger 8 Light Sheet based Fluorescence Microscopy Emmanuel G. Reynaud 9 Macrophotography Eric Roettinger 10 Electron Microscopy in Marine Biology To be defined Glossary Index