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9780813820859

Industrial Bioseparations Principles and Practice

by
  • ISBN13:

    9780813820859

  • ISBN10:

    0813820855

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2008-07-01
  • Publisher: Wiley-Blackwell
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Supplemental Materials

What is included with this book?

Summary

Industrial Bioseparations offers comprehensive coverage of bioseparations including all unit operations. This new book offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today. It is laid out in a methodical way with preliminary chapters covering general approaches to bioseparations for commercially important biomacromolecules, thermodynamics and mass transfer principles, and following chapters addressing unit operations such as filtration and chromatography. Lab experiments are included which emphasize obtaining scale up parameters as well as commonly used operating conditions.

Author Biography

Daniel Forciniti, Ph.D is a Professor at the Department of Chemical Engineering at the University of Missouri, Rolla. Dr. Forciniti joined the University of Missouri, Rolla after receiving his Ph.D. from North Carolina State University in 1991. Dr. Forciniti has published and made presentations extensively on chromatography, biomacromolecules, and biotechnology, including peer-reviewed articles, contributed chapters, and invited lectures.

Table of Contents

Preface
Introduction to Bioseparations
Statement of the problem
Engineering Developments
Current approaches to bioseparations
Thermodynamics
A brief introduction
Thermodynamics of open systems
Thermodynamic analysis of processes
Principles of Mass Transfer
Diffusion
Fluxes
Law of conservation of mass
Examples
General Properties of Bioproducts
Proteins
Structure
Stability (solubility and aggregation)
Separation of complex protein mixtures by two-dimensional electrophoresis
Nucleic Acids
Structure
Properties
Laboratory experiments
Cell disruption
General approach to cell disruption
Types of cells
Location of product
Physical Chemistry Properties of Cells (as they relate to disruption)
Bead Mills
Homogenizers
Chemical Methods
Biochemical Methods
Laboratory experiments
Separation of Solids
General Principles
Flocculation of proteins
Inclusion bodies
Filtration
Conventional Filtration
Ultrafiltration
Centrifugation
Sedimentation
Laboratory experiments
Liquid/liquid extraction
Chromatography
General Principles
Gel permeation
Ion Exchange Chromatography
Hydrophobic Interactions Chromatography
Affinity Chromatography
Simulated Moving Bed Chromatography
Laboratory Experiments
Protein Precipitation
Precipitation by salting-out
Isoelectric Precipitation
Precipitation by non-ionic polymers
Laboratory Experiments
Polishing
Freeze drying
Crystallization
Product Formulation
Laboratory Experiments
Separations in a Chip
Introduction to micro-devices
Fluidics
Micro-reactors, micro-mixers, and valves
Chromatography and electrophoresis in a chip
Micro-sensors
Design of Separation cascades
General Principles
Examples
Laboratory Projects
Appendix
Experimental Design
Introduction to Experimental Design
Precision and Accuracy
Descriptive Statistics
Statistical Tests
Examples
Table of Contents
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

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