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Written for students and researchers in systems biology, the second edition of this best-selling textbook continues to offer a clear presentation of design principles that govern the structure and behavior of biological networks, highlighting simple, recurring circuit elements that make up the network. Rigorously classroom-tested, it contains new additions as well as corrections and revisions for better flow. This edition includes four new chapters:Perfect Robustness, Scaling in Development, Noise and Variability in Biological Circuits,and Evolution of Modularity. It also doubles the number of exercises and adds an appendix.