9780824707262

Isolation and Purification of Proteins

by ;
  • ISBN13:

    9780824707262

  • ISBN10:

    0824707265

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2003-02-05
  • Publisher: CRC Press

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Summary

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Table of Contents

Prefacep. iii
Contributorsp. vii
Introductionp. ix
Release of Protein from Biological Hostp. 1
Chromatography: The High-Resolution Technique for Protein Separationp. 29
Selectivity in Affinity Chromatographyp. 57
Genetic Approaches to Facilitate Protein Purificationp. 95
Solid-Liquid Separationp. 131
Membranes for Protein Isolation and Purificationp. 191
Precipitation of Proteins: Nonspecific and Specificp. 225
Protein Crystallization for Large-Scale Bioseparationp. 277
Extraction for Rapid Protein Isolationp. 321
Adsorption as an Initial Step for the Capture of Proteinsp. 373
Fast Chromatography of Proteinsp. 423
Novel Approaches to the Chromatography of Proteinsp. 455
Scale-up and Commercial Production of Recombinant Proteinsp. 503
Process Monitoring During Protein Purificationp. 515
Protein Formulationp. 549
Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purificationp. 605
Indexp. 623
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