Lab Manual and Workbook in Microbiology: Applications to Patient Care

by Morello, Josephine; Granato, Paul; Mizer, Helen

• ISBN13: 9780073522531
• ISBN10: 0073522538
• Edition: 10th
• Format: Spiral Bound
• Copyright: 2010-08-20
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

The emphasis of this lab manual is on the basic principles of diagnostic microbiology for students preparing to enter an allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and to practice safety in the laboratory and hospital environment. It remains oriented primarily toward meeting the interests and needs of those who will be directly involved in patient care and who wish to learn how microbiological principles should be applied in the practice of their professions.The authors have emphasized the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. The exercises illustrate as simply as possible the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilization. Attention is given to the role of the health professional in regard to appropriate collection of clinical specimens and the applications of aseptic and disinfectant techniques as they relate to patient care.

Table of Contents

Basic Techniques of Microbiology

The Microscope

Handling and Examining Cultures

Hanging Drop and Wet-Mount Preparations

Simple Stains

Gram Stain

Acid-Fast Stain

Special Stains

Culture Media

Streaking Technique to Obtain Pure Cultures

Pour-Plate and Subculture Techniques

Culturing Microorganisms from the Environment

Destruction of Microorganisms

Moist and Dry Heat

The Autoclave

Disinfectants

Antimicrobial Agent Susceptibility Testing and Resistance

Diagnostic Microbiology in Action

Primary Media for Isolation of Microorganisms

Some Metabolic Activities of Bacteria

Activities of Bacterial Enzymes

Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms.

Staphylococci

Streptococci, Pneumococci, and Enterococci

Haemophilus, Corynebacteria, and Bordetella

Clinical Specimens from the Respiratory Tract

The Enterobacteriaceae (Enteric Bacilli)

Clinical Specimens from the Intestinal Tract

Urine Culture Techniques

Neisseria and Spirochetes

Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients’ Antibodies

Anaerobic Bacteria

Mycobacteria

Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions

Fungi: Yeast and Molds

Protozoa and Animal Parasites

Serodiagnosis of Infectious Disease

Applied (Sanitary) Microbiology

Bacteriological Analysis of Water

Bacteriological Analysis of Milk

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