The Microbiology Laboratory Manual by Pollack presents exercises and experiments on microbiology laboratory. The labs are introduced in a clear and concise manner, while maintaining a reader-friendly tone. The manual contains a variety of interactive activities and experiments that teach the basic concepts of microbiology. It also covers methods that allow the safe movement or transfer of microbial cells from one type of growth environment, classification and identification of microbes, microbial biochemistry, medical, food and environmental microbiology.
Introduction: Laboratory Operations and Safety.
PART I: GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE.
1 Laboratory Safety: Introduction to the Microscope.
2 Transfer and Isolation Techniques Microbes in the Environment.
PART II MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS.
3 Cultural and Cellular Morphology.
4 Bacterial Growth.
5 Gram Stain Acid-Fast Stain.
6 Endospore Stain Capsule Stain Hanging Drop Technique.
8 Viruses-Visualization and Enumeration.
PART III MICROBIAL CONTROL AND BIOCHEMISTRY.
10 Microbial Sensitivity Testing Ultraviolet Light Sensitivity.
11 Bacterial Biochemistry.
12 Gas Requirements of Microorganisms.
13 Specialized Media.
PART IV MEDICAL MICROBIOLOGY.
16 Speciman-Handling Protocols.
17 Specific Laboratory Tests.
19 Identification of Enteric Pathogens: Traditional Methods.
20 Identification of EntericPathogens: Rapid Identification Methods.
21 Identification of a Bacterial Unknown: The Gram-Negative Unknown.
22 Identification of a Bacterial.
PART V FOOD AND ENVIRONMENTAL MICROBIOLOGY.
23 Identification and Quantitation of Microbial Numbers in a Water Sample.
24 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial.
25 Soil Microbiology.
26 Microbial Ecology.
Appendix 1 Flow Chart for the Identification of Enterobacteriaceae.
Appendix 2 Gram-Negative Flow Chart.
Appendix 3 Gram-Positive Flow Chart.