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Laboratory Experiments in Microbiology : An Introduction,9780805375893
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Laboratory Experiments in Microbiology : An Introduction

by ;
Edition:
6th
ISBN13:

9780805375893

ISBN10:
0805375899
Format:
Spiral Bound
Pub. Date:
1/1/2001
Publisher(s):
Benjamin Cummings

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Summary

Newly revised to accompany Microbiology: An Introduction, Seventh Edition by Tortora, Funke, and Case, this lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology. Intended as a manual of basic microbiologic techniques, this popular lab manual features applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various preprofessional programs. Experiments have been refined in this new edition to encourage students to develop critical thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included whenever possible, and increased emphasis is placed on lab safety.

Table of Contents

Introduction 1(4)
part one Microscopy 5(22)
Use and Care of the Microscope
7(10)
Examination of Living Microorganisms
17(10)
part two Staining Methods 27(40)
Preparation of Smears and Simple Staining
29(6)
Negative Staining
35(4)
Gram Staining
39(6)
Acid-Fast Staining
45(6)
Structural Stains (Endospore, Capsule, and Flagella)
51(10)
Morphologic Unknown
61(6)
part three Cultivation of Bacteria 67(30)
Microbes in the Environment
69(8)
Transfer of Bacteria: Aseptic Technique
77(8)
Isolation of Bacteria by Dilution Techniques
85(8)
Special Media for Isolating Bacteria
93(4)
part four Microbial Metabolism 97(42)
Carbohydrate Catabolism
99(6)
Fermentation of Carbohydrates
105(8)
Protein Catabolism, Part 1
113(6)
Protein Catabolism, Part 2
119(6)
Respiration
125(4)
Rapid Identification Methods
129(10)
part five Microbial Growth 139(24)
Oxygen and the Growth of Bacteria
141(8)
Determination of a Bacterial Growth Curve: The Role of Temperature
149(6)
Other Influences on Microbial Growth: Osmotic Pressure and pH
155(8)
part six Control of Microbial Growth 163(30)
Physical Methods of Control: Heat
165(6)
Physical Methods of Control: Ultraviolet Radiation
171(6)
Chemical Methods of Control: Disinfectants and Antiseptics
177(4)
Chemical Methods of Control: Antimicrobial Drugs
181(8)
Effectiveness of Hand Scrubbing
189(4)
part seven Microbial Genetics 193(42)
Regulation of Gene Expression
195(8)
Isolation of Bacterial Mutants
203(6)
Transformation of Bacteria
209(4)
DNA Fingerprinting
213(6)
Genetic Engineering
219(10)
Ames Test for Detecting Possible Chemical Carcinogens
229(6)
part eight The Microbial World 235(32)
Unknown Identification and Bergey's Manual
237(6)
Fungi: Yeasts
243(6)
Fungi: Molds
249(8)
Phototrophs: Algae and Cyanobacteria
257(6)
Protozoa
263(4)
part nine Viruses 267(16)
Isolation and Titration of Bacteriophages
269(8)
Plant Viruses
277(6)
part ten Interaction of Microbe and Host 283(16)
Epidemiology
285(8)
Koch's Postulates
293(6)
part eleven Immunology 299(26)
Nonspecific Resistance
301(8)
Blood Group Determination: Slide Agglutination
309(6)
Agglutination Reactions: Microtiter Agglutination
315(4)
ELISA Technique
319(6)
part twelve Microorganisms and Disease 325(40)
Bacteria of the Skin
327(6)
Bacteria of the Respiratory Tract
333(8)
Bacteria of the Mouth
341(6)
Bacteria of the Gastrointestinal Tract
347(8)
Bacteria of the Urogenital Tract
355(6)
Identification of an Unknown from a Clinical Sample
361(4)
part thirteen Microbiology and the Environment 365(42)
Microbes in Water: Multiple-Tube Technique
367(8)
Microbes in Water: Membrane Filter Technique
375(4)
Microbes in Food: Contamination
379(6)
Microbes Used in the Production of Foods
385(4)
Microbes in Soil: The Nitrogen and Sulfur Cycles
389(12)
Microbes in Soil: Bioremediation
401(6)
Appendices 407(26)
A Pipetting
409(2)
B Dilution Techniques and Calculations
411(4)
C Use of the Spectrophotometer
415(2)
D Graphing
417(4)
E Use of the Dissecting Microscope
421(2)
F Use of the Membrane Filter
423(2)
G Electrophoresis
425(2)
H Keys to Bacteria
427(6)
Index 433


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