Mass Spectrometry: Instrumentation, Interpretation, and Applications

by Editor: Rolf Ekman; Editor: Jerzy Silberring; Editor: Ann M. Brinkmalm; Dominic M. Desiderio (University of Tennessee Health Science Center); Nico M. Nibbering (Vrije Universiteit in Amsterdam)

• ISBN13: 9780470395806
• ISBN10: 047039580X
• Format: eBook
• Copyright: 2008-12-01
• Publisher: Wiley

Summary

With contributions from noted experts from Europe and North America, Mass Spectrometry Instrumentation, Interpretation, and Applications serves as a forum to introduce students to the whole world of mass spectrometry and to the many different perspectives that each scientific field brings to its use. The book emphasizes the use of this important analytical technique in many different fields, including applications for organic and inorganic chemistry, forensic science, biotechnology, and many other areas. After describing the history of mass spectrometry, the book moves on to discuss instrumentation, theory, and basic applications.

Table of Contents

Foreword

Contributors

Instrumentation

Definitions and Explanations

References

A Mass Spectrometer's Building Blocks

Ion Sources

Mass Analyzers

Detectors

References

Tandem Mass Spectrometry

Tandem MS Analyzer Combinations

Ion Activation Methods

References

Separation Methods

Chromatography

Electric-Field Driven Separations

References

Interpretation

Introduction to Mass Spectra Interpretation: Organic Chemistry

Basic Concepts

Inlet Systems

Physical Bases of Mass Spectrometry

Theoretical Rules and Approaches to Interpret Mass Spectra

Practical Approaches to Interpret Mass Spectra

References

Sequencing of Peptides and Proteins

Basic Concepts

Tandem Mass Spectrometry of Peptides and Proteins

Peptide Fragmentation Nomenclature

Technical Aspects and Fragmentation Rules

Why Peptide Sequencing?

De Novo Sequencing

Peptide Derivatization Prior to Fragmentation

Acknowledgments

References

Online Tutorials

Optimizing Sensitivity and Specificity in Mass Spectrometric Proteome Analysis

Quantitation

Peptide and Protein Identification

Success Rate and Relative Dynamic Range

Summary

References

Applications

Doping Control

References

Oceanography

References

"Omics" Applications

Introduction

Genomics and Transcriptomics

Proteomics

Metabolomics

SPace Sciences

Introduction

Origins

Dynamics

The Space MS Paradox

A Brief History of Space MS

GENESIS and the Future

References

Bioterrorism

What is Bioterrorism?

Some Historical Accounts of Bioterrorism

Geneva Protocol of 1925 and Biological Weapons Convention of 1972

Categories of Biothreat Agents

Challenges

MS Identification of Biomarker Proteins

Development of New Therapeutics and Vaccines Using Immunoproteomics

References

Imaging of Small Molecules

SIMS Imaging

Biological Applications (Cells, Tissues, and Pharmaceuticals)

Catalysis

Forensics

Semiconductors

The Future

References

Utilization of Mass Spectrometry in Clinical Chemistry

Introduction

Where are Mass Spectrometers Utilized in Clinical Applications?

Most Common Analytes Detected by Mass Spectrometers

Multianalyte Detection of Clinical Biomarkers, The Real Success Story

Quantitative Profiling

A Clinical Example of the Use of Mass Spectrometry

Demonstrations of Concepts of Quantification in Clinical Chemistry

Polymers

Introduction

Instrumentation, Sample Preparation

Table of Contents provided by Publisher. All Rights Reserved.