RNA Helicases

by Jankowsky

• ISBN13: 9780123965462
• ISBN10: 0123965462
• Edition: 1st
• Format: Hardcover
• Copyright: 2012-06-22
• Publisher: Elsevier Science

Summary

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes.

Table of Contents

Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy

Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods

Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases

Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer

RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases

Molecular Mechanics of RNA Translocases

Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping

Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase

Structural Analysis of RNA Helicases with Small-angle X-ray Scattering

Analysis of Co-factor Effects on RNA Helicases

Analysis of DEAD-box Proteins in mRNA Export

Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay

Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)

In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly

Analysis of RNA Helicases in P-bodies and Stress Granules

DEAD box RNA Helicases as Transcription Co-factors

DEAD-box RNA Helicases in Gram-positive RNA-decay

RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches

Determination of Host RNA Helicases Activity in Viral Replication

Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A

Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase

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