9780123965462

Methods in Enzymology

by
  • ISBN13:

    9780123965462

  • ISBN10:

    0123965462

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2012-06-22
  • Publisher: Academic Pr

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Supplemental Materials

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Summary

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes.

Table of Contents

Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy
Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods
Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases
Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer
RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases
Molecular Mechanics of RNA Translocases
Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping
Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase
Structural Analysis of RNA Helicases with Small-angle X-ray Scattering
Analysis of Co-factor Effects on RNA Helicases
Analysis of DEAD-box Proteins in mRNA Export
Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay
Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)
In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly
Analysis of RNA Helicases in P-bodies and Stress Granules
DEAD box RNA Helicases as Transcription Co-factors
DEAD-box RNA Helicases in Gram-positive RNA-decay
RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches
Determination of Host RNA Helicases Activity in Viral Replication
Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A
Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase
Table of Contents provided by Publisher. All Rights Reserved.

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