9780121822378

Microbial Growth in Biofilms, Part A: Developmental and Molecular Biological Aspects

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  • ISBN13:

    9780121822378

  • ISBN10:

    0121822370

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2001-05-25
  • Publisher: Elsevier Science
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Summary

This volume and its companion, Volume 337, supplement Volume 310,. These volumes provide a contemporary sourcebook for virtually any kind of experimental approach involving biofilms. They cover bioengineering, molecular, genetic, microscopic, chemical, and physical methods.

Table of Contents

Contributors to Volume 336
Preface
Volume in Series
Developmental Processes in Biofilms
Whole Genome DNA Microarray Expression Analysis of Biofilm Development by Vibrio cholerae Ol El Torp. 3
Biofilm Formation as a Developmental Processp. 19
Signaling and Gene Expression in Biofilm
Surface Sensing, Swarmer Cell Differentiation, and Biofilm Developmentp. 29
Acylated Homoserine Lactone Detection in Pseudomonas aeruginosa Biofilms by Radiolabel Assayp. 41
Genetic and Phenotypic Analysis of Multicellular Behavior in Salmonella typhimuriump. 48
Gene Transfer in Bacterial Biofilmsp. 60
Conversion to Mucoidy in Pseudomonas aeruginosa Infecting Cystic Fibrosis Patientsp. 60
Subtractive Hybridization-Based Identification of Genes Uniquely Expressed or Hyperexpressed during Biofilm Growthp. 76
Biofilm-Induced Gene Expression and Gene Transferp. 84
Directed Movement and Surface-Borne Motility of Myxococcus and Pseudomonasp. 94
A General Method to Identify Bacterial Genes Regulated by Cell-to-Cell Signalingp. 102
Genetic and Chemical Tools for Investigating Signaling Processes in Biofilmsp. 108
In Situ Quantification of Gene Transfer Biofilmsp. 129
Transcriptional Analysis of Genes Involved in Pseudomonas aeruginosa Biofilmsp. 144
First Stages of Biofilm Formation: Characterization and Quantification of Bacterial Functions Involved in Colonization Processp. 152
Growth of Bacteriophage in Bacteria in Biofilms
Phenotype Characterization of Genetically Defined Microorganisms and Growth of Bacteriophase in Biofilmsp. 163
Biofilms of Staphylococci
Methods for Studying Biofilms Produced by Staphylococcus epidermidisp. 177
Methods to Detect and Analyze Phenotypic Variation in Biofilm-Forming Staphylococcip. 195
In Vivo Models to Evaluate Adhesion and Biofilm Formation by Staphylococcus epidermidisp. 206
Genetic and Biochemical Analysis of Staphylococcus epidermidis Biofilm Accumulationp. 215
In Vitro Methods to Study Staphylococcal Biofilm Formationp. 239
Efficient RNA Isolation Method for Analysis of Transcription in Sessile Staphylococcus epidermidis Biofilm Culturesp. 255
Metabolic Potential of Biofilms
Assessment of Metabolic Potential of Biofilm-Associated Bacteriap. 265
Extracellular Polymers
Characterization of Extracellular Citinolytic Activity in Biofilmsp. 279
Isolation and Biochemical Characterization of Extracellular Polymeric Substances from Pseudomonas aeruginosap. 302
Microbiological Aspects of Microbial Biofilm
Approach to Analze Interactions of Microorganisms. Hydrophobic Substrates, and Soil Colloids Leading to Formation of Composite Biofilms, and to Study Initial Events in Microbiogeological Processesp. 317
Extracellular Polymers of Microbial Communities Colonizing Acient Limestone Monumentsp. 331
Studying Phototrophic and Heterotrophic Microbial Communities on Stone Monumentsp. 340
Identification of Archaea in Objects of Art by Denaturing Gradient Gel Electrophoresis Analysis and Shotgun Cloningp. 356
Probiotics
Lactobacilli as Vehicles for Targeting Antigens to Mucusal Tissues by Surface Exposition of Foreign Antigensp. 369
A Dot-Blot Assay for Adhesive Components Relative to Probioticsp. 389
Understanding Urogenital Biofilms and Potential Impact of Probioticsp. 403
Surface Characterization and Adhesive Properties of Bifidobacteriap. 411
Author Indexp. 429
Subject Indexp. 457
Table of Contents provided by Blackwell. All Rights Reserved.

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