Microbiology A Photographic Atlas for the Laboratory

by Alexander, Steven K., Ph.D.; Strete, Dennis

• ISBN13: 9780805327328
• ISBN10: 0805327320
• Edition: 1st
• Format: Spiral Bound
• Copyright: 2000-07-18
• Publisher: Pearson

Summary

Tailored for the introductory microbiology laboratory course, the book features approximately 400 color photographs that demonstrate the results of laboratory procedures and show the morphology of important microorganisms. The photographs demonstrate the unique characteristics of common microorganisms and also their appearance after various stains and tests. Many of the photographs are labeled to point out important structures. The clearly written descriptions accompanying the photos helps students understand and, in some cases, achieve the results depicted in the photographs. Each laboratory procedure has the following sections: a Purpose and Procedure Summary, Tips for Success, and Expected Results. For anyone interested in microorganisms.

Table of Contents

Microscopes and Their Use in the Study of Cells and Viruses	1	(10)
Bright-Field Microscope	1	(3)
Fluorescent Microscope	4	(1)
Electron Microscope	5	(6)
Scanning Electron Microscope (SEM)	5	(1)
Transmission Electron Microscope (TEM)	5	(6)
Microscopic Observation of Bacteria, Fungi, and Protozoa	11	(22)
Bacteria	12	(6)
Size	12	(1)
Cell Shapes	12	(2)
Cell Arrangements	14	(3)
Actinomycetes	17	(1)
Using Wet Mounts to Observe Bacteria	18	(1)
Fungi	19	(9)
Growth Forms and Reproduction	19	(1)
Divisions of Fungi	19	(6)
Using Wet Mounts to Observe Fungi	25	(3)
Protozoa	28	(5)
Protozoan Groups	28	(5)
Staining Bacteria	33	(14)
Preparing Cells for Staining	33	(1)
Morphological Stains	34	(1)
Simple Stain	34	(1)
Negative Stain	35	(1)
Differential Stains	35	(1)
Gram Stain	35	(5)
Acid-Fast Stain	40	(1)
Structural Stains	41	(1)
Capsule Stain	42	(1)
Endospore Stain	42	(3)
Flagella Stain	45	(2)
Cultivation of Bacteria and Fungi	47	(22)
Sample Collection	47	(2)
Environmental Samples	47	(1)
Clinical Samples	47	(2)
Incubation of Inoculated Media	49	(2)
Temperature Considerations	49	(1)
Atmospheric Considerations	50	(1)
Examination of Growth on Agar Media	51	(10)
Growth of Organisms from the Environment	51	(2)
Growth of Organisms from Clinical Samples	53	(8)
Examination of Growth in Broth	61	(1)
Colony Selection	62	(1)
Isolation by the Streak-Plate Method	63	(5)
Maintenance of Bacterial Cultures	68	(1)
Biochemical Tests for the Identification of Bacteria	69	(24)
Carbohydrate Utilization	69	(2)
Catalase Test	71	(1)
Citrate Utilization	72	(1)
Decarboxylase Tests	73	(1)
Denitrification	74	(1)
Gelatin Utilization	75	(1)
Hydrogen Sulfide (H2S) Production	76	(1)
Indole Production	77	(1)
Litmus Milk Reactions	78	(2)
Methyl Red Test	80	(1)
Motility Test	81	(1)
Oxidase Test	82	(1)
Oxidation-Fermentation (O-F) Test	83	(2)
Phenylalanine Deaminase Test	85	(1)
Skim Milk Utilization	86	(1)
Starch Hydrolysis Test	87	(1)
Triple Sugar Iron (TSI) Agar and Kligler Iron Agar (KIA) Tests	88	(2)
Urea Utilization	90	(1)
Voges-Proskauer Test	91	(2)
Bacterial Identification Methods	93	(8)
Identification Using Conventional Methods	93	(3)
Identification Using Multitest Systems	96	(2)
Identification Using Automated Methods	98	(3)
Evaluating Antibacterial Chemical Agents	101	(10)
Evaluating Handwashing Effectiveness	101	(1)
Evaluating Antiseptics and Disinfectants: The Filter Paper Method	102	(2)
Antibiotic Effectiveness	104	(1)
Evaluating Antibiotics: The Kirby-Bauer Method	105	(3)
Evaluating Antibiotics: Etest®	108	(1)
Antibiotic Resistance	109	(2)
Soil and Water Microbiology	111	(10)
Microbial Enumeration By Plate Count	111	(5)
Most Probable Number (MPN) Method	116	(4)
Membrane Filter Method	120	(1)
Survey of Microbial Pathogens	121	(24)
Bacterial Pathogens	121	(13)
Fungal Pathogens	134	(4)
Protozoan Pathogens	138	(7)
Arthropod Vectors of Human Diseases	145	(6)
Vectors of Human Diseases	146	(5)
Acarines	146	(2)
Insects	148	(3)
Multicellular Parasites	151	(20)
Basic Structural Characteristics of Multicellular Parasites	151	(4)
Life Cycles of Multicellular Parasites	155	(16)
Phylum Platyhelminthes (Flatworms)	155	(11)
Phylum Nematoda (Roundworms)	166	(5)
Clinical Applications of Host Responses to Infection	171	(16)
Indentifying White Blood Cell Types	171	(3)
White Blood Cell Counts	174	(2)
Organs and Tissues of the Immune System	176	(3)
Immunization	179	(1)
Antigen-Antibody Precipitation Reactions in Solution	179	(2)
Immunodiffusion Methods	181	(2)
Agglutination Reactions	183	(1)
Enzyme-Linked Immunosorbent Assay (ELISA)	184	(1)
SDS-Polyacrylamide Gel Electrophoresis and Western Blot	185	(2)
Index	187