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Written by world experts in the field of nanotechnology for nucleic acid (NA) delivery, the authors explore the state of the art in delivery strategies using lipids, polymers, chemical conjugates, NA aptamers, and proteins. They strongly emphasize issues and aspects that are of essence to the pharmaceutical industry working in areas such as stability, general toxicity, immune-toxicity, pharmacokinetics, and the efficacy and validation of new drug targets in vivoby using unique approaches based on exquisite nanotechnology strategies. The contributing authors are world leaders in their respective fields and founders of different approaches.