9780521022149

Practical Exercises in Parasitology

by
  • ISBN13:

    9780521022149

  • ISBN10:

    0521022142

  • Edition: 1st
  • Format: Paperback
  • Copyright: 2005-11-03
  • Publisher: Cambridge University Press
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  • The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

Summary

Parasitology is an immensely important aspect of biological science. This manual presents fifty easy-to-follow laboratory exercises for student practical (lab) classes. All the exercises are tried and tested by the authors and are used in a wide variety of university undergraduate teaching departments. They range from relatively simple observational exercises, using local materials and requiring little in the way of equipment, to more technically demanding experiments in physiology and molecular parasitology. Each exercise includes a list of necessary equipment, consumables and sources of parasite material, instructions for staff and students, including aspects of safety, expected results, and some analysis provided by questions. In addition, the text also includes ideas for further exploration and information on similar exercises, as well as lists of selected further reading. This book should be an essential purchase for all teachers of parasitology at the university undergraduate level and for students taking laboratory practical classes in the subject.

Table of Contents

List of contributors
xi
Preface xvii
General advice 1(10)
Observational Exercises on Parasites
Local wild and domestic hosts as sources of parasites
Parasites of the earthworm: Monocystis (Protozoa) and Rhabditis (Nematoda)
11(8)
D. Wakelin
D. I. De Pomerai
J. M. Behnke
Parasites of marine molluscs (Littorina)
19(12)
R. E. B. Hanna
D. W. Halton
Parasites of fish: Whiting and Diclidophora merlangi (Monogenea)
31(6)
D. W. Halton
Parasites of fish: Plaice/flounder and Lepeophtheirus pectoralis (Copepoda)
37(8)
P. A. Heuch
T. A. Schram
Parasites of domestic livestock: Pig and Ascaris suum (Nematoda)
45(8)
J. M. Behnke
Parasites of domestic livestock: Sheep and Fasciola hepatica (Trematoda)
53(10)
C. E. Bennett
Parasites of crops: Potato cyst nematode (PCN) Globodera pallida (Nematoda)
63(10)
J. T. Jones
Laboratory maintained species
Protozoan parasites of the intestinal tract of the cockroach, Periplaneta americana
73(4)
J. E. Williams
D. C. Warhurst
Protozoan parasites of the mouse intestinal tract
77(4)
J. E. Williams
D. C. Warhurst
Rodent malaria
81(8)
J. E. Smith
Malaria: an example of a vector transmitted parasite
89(8)
H. Hurd
R. E. Sinden
Larval and adult Echinostoma spp. (Trematoda)
97(10)
B. Fried
Schistosoma mansoni (Trematoda)
107(8)
M. J. Doenhoff
L. H. Chappell
J. M. Behnke
Hymenolepis diminuta (Cestoda)
115(8)
J. M. Behnke
Heligmosomoides polygyrus (Nematoda)
123(10)
J. M. Behnke
L. H. Chappell
A. W. Pike
Ecology
Pinworms (Nematoda, Oxyuroidea) in the American cockroach, Periplaneta americana
133(8)
W. M. Hominick
J. M. Behnke
Distribution and microhabitat of a monogenean on the gills of mackerel
141(6)
C. R. Kennedy
Population dynamics of Gyrodactylus on stickleback
147(14)
R. C. Tinsley
Intraspecific competition in the cestode Hymenolepis diminuta, in rats
161(6)
C. R. Kennedy
J. M. Behnke
Transmission dynamics and the pattern of dispersion of the cestode Hymenolepis diminuta, in the intermediate host population
167(8)
C. R. Kennedy
J. M. Behnke
Physiology and Biochemistry
Hatching in vitro of oncospheres/hexacanth larvae of Hymenolepis diminuta
175(8)
C. E. Bennett
Activation of the cysticercoids of Hymenolepis species in vitro
183(8)
J. M. Behnke
Membrane transport in the cestode Hymenolepis diminuta, in vitro
191(10)
C. Arme
Glycogen utilisation and deposition in flatworm parasites
201(8)
D. W. Halton
Effects of classical transmitters on the motility of parasitic roundworms and flatworms
209(10)
A. G. Maule
N. J. Marks
J. W. Bowman
Electrophysiology of Ascaris suum body muscle
219(12)
R. J. Martin
Immunocytochemical localisation of neuroactive substances in helminth parasites
231(12)
N. J. Marks
A. G. Maule
D. W. Halton
Pathology and Immunology
Encapsulation of foreign matter (not-self) by earthworms
243(6)
D. Wakelin
D. I. De Pomerai
J. M. Behnke
Opsonisation of trypanosomes
249(6)
C. M. R. Turner
Production and screening of monoclonal antibodies against Leishmania promastigotes
255(12)
M. Hommel
M. L. Chance
Pathological effects of Mesocestoides corti and Schistosoma mansoni
267(10)
J. Chernin
Quantification of lymphocyte populations in the spleen and thymus
277(6)
J. Chernin
Use of basic indirect ELISA for the detection of antibodies produced by experimental immunisation
283(10)
D. A. Johnson
C. Mcguire
SDS PAGE and Western blotting for the detection of antibodies produced by experimental immunisation
293(12)
C. Mcguire
D. A. Johnson
Chemotherapy
Sensitivity of a coccidial parasite, Eimeria, to an ionophore, monensin
305(8)
M. W. Shirley
Egg hatch assay for determination of resistance of nematodes to benzimidazole anthelmintics
313(8)
F. Jackson
E. Jackson
R. L. Coop
Larval migration inhibition assay for determination of susceptibility of nematodes to levamisole
321(8)
F. Jackson
E. Jackson
R. L. Coop
Effect of anthelmintics on nematodes
329(6)
D. L. Lee
J. E. Smith
Molecular Parasitology
Purification of DNA
335(8)
P. A. Bates
T. Knapp
J. M. Crampton
DNA digestion and gel electrophoresis
343(6)
P. A. Bates
T. Knapp
J. M. Crampton
Restriction enzyme mapping
349(8)
P. A. Bates
T. Knapp
J. M. Crampton
Construction of a genomic library
357(8)
P. A. Bates
T. Knapp
J. M. Crampton
Detection and differentiation of Entamoeba histolytica and E. dispar by PCR
365(10)
J. E. Williams
D. Britten
Differentiation between parasite species by agglutination and detection of parasite surface carbohydrates, using non-conjugated lectins
375(10)
G. A. Ingram
Tentative identification of parasite and tissue surface carbohydrates by conjugated lectins
385(12)
G.A. Ingram
Behaviour
Behaviour of the miracidia of Fasciola hepatica and demonstration of other larval stages
397(10)
C. E. Bennett
Effects of age and environmental factors on the swimming behaviour of the cercariae of Cryptocotyle lingua (Trematoda)
407(8)
J. G. Rea
S. W. B. Irwin
Changes in host behaviour as a consequence of parasite infection
415(8)
H. Hurd
Behaviour of the amphipod Gammarus pulex, infected with cystacanths of acanthocephalans
423(10)
D. B. A. Thompson
A. F. Brown
Effects of Schistocephalus solidus (Cestoda) on stickleback feeding behaviour
433(12)
I. Barber
Appendix 1 Reagent index 445(2)
Appendix 2 UK suppliers 447(3)
Appendix 3 US suppliers 450(2)
Index 452

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