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Each chapter focuses on the principles, applications, and limitations of a different technology. Fostering active engagement with the techniques presented, each chapter uses worked examples and problems to encourage students to critically appraise scientific data and select the most appropriate methods when designing an experimental strategy to undertake their own research.
* Provides a clear overview of contemporary biomolecular techniques, making it the ideal resource for students and early-stage researchers
* Uses examples throughout to illustrate how each technique is applied, and shows how the data yielded can be analyzed and understood
* Emphasizes the pros and cons of the techniques presented, helping students to evaluate the experimental options at their disposal
* Worked examples encourage students to become confident with data handling--a central part of any experimental research
* A Companion Website provides a range of additional resources
Table of Contents
1. Gene Cloning Essentials, David Hornby and Qaiser I. Sheikh
2. PCR, Martin Evison
3. DNA Mutagenesis, Sarah E. Deacon and Michael J. McPherson
4. DNA Sequencing, Tania Slatter and Alison Fitches
5. Measuring DNA/Protein Interactions, Luis Acevedo, Ana Sanz, Paul Labhart, and Mary Anne Jelinek
6. RNA Interference Technology, Gavin Batman, Thomas Walker, and Ian Hampson
7. Recombinant Protein Expression, Michael Harrison and Michael J. McPherson
8. Protein Purification, Iain Manfield
9. Antibody Technology, Gavin Allsop and John Colyer
10. Measuring Protein-Protein Interactions: Qualitative Approaches, Johanna M. Avis and Sanjay Nilapwar
11. Measuring Protein-Protein Interactions: Quantitative Approaches, Johanna M. Avis and Sanjay Nilapwar
12. Structural Analysis of Proteins: X-ray Crystallography, Thomas Edwards, Arwen Pearson, Gary Thompson, Arnout Kalverda, Oliver Farrance, David Brockwell, and Gareth Morgan
13. Mass Spectrometry, James R. Ault and Alison E. Ashcroft
14. Proteomic Analysis, Richard Unwin
15. Culturing Mammalian Cells, Geoffrey J. Pilkington, Suzanne M. Birks, and Laura K. Donovan
16. Flow Cytometry, John Lawry
17. Bioimaging: Light and Electron Microscopy, Gareth Howell and Kyle Dent
18. Histopathology in Biomolecular Research, Noelyn Hung and Tania Slatter
19. Mouse Models in Scientific Research, Brian Corbett and Jeannie Chin
20. Mathematical Models in Biomolecular Sciences, José A. García