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Ultra-High Performance Liquid Chromatography and Its Applications,9780470938423
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Ultra-High Performance Liquid Chromatography and Its Applications



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This is the 1st edition with a publication date of 4/15/2013.
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This is the first resource to fully cover the instrumentation, method development, and applications of Ultra-High Performance Liquid Chromatography (U-HPLC). It details both the benefits and limitations of this method in the pharmaceutical industry, clinical research, the food industry, and environmental services. It covers U-HPLC topics coupled with ultra-violet detector (UV) and mass spectrometer (MS), instrumentation frequently used in solving complex molecules. This is an essential reference for scientists who utilize chromatographic techniques, including in academia, as well as pharmaceutical, biotechnology, chemical, food, environmental, and related industries.

Author Biography

QUANYUN ALAN XU, PhD, is a Senior Research Scientist in the Pharmacology and Analytical Laboratory Facility at the University of Texas MD Anderson Cancer Center. Dr. Xu is the coauthor of Analytical Methods for Therapeutic Drug Monitoring and Toxicology as well as more than seventy scientific papers and book chapters.

Table of Contents

Preface vii

Contributors ix

1. UHPLC Method Development 1
Shujun Chen and Alireza Kord

2. Method Transfer Between HPLC and UHPLC Platforms 31
Gregory K. Webster, Thomas F. Cullen, and Laila Kott

3. Practical Aspects of Ultrahigh Performance Liquid Chromatography 55
Naijun Wu, Christopher J. Welch, Theresa K. Natishan, Hong Gao, Tilak Chandrasekaran, and Li Zhang

4. Coupling UHPLC with MS: The Needs, Challenges, and Applications 95
Julie Schappler, Serge Rudaz, Jean-Luc Veuthey, and Davy Guillarme

5. The Potential of Shell Particles in Fast Liquid Chromatography 133
Szabolcs Fekete and Jeno Fekete

6. UHPLC Determination of Drugs of Abuse in Human Biological Matrices 169
Fabio Gosetti, Eleonora Mazzucco, and Maria Carla Gennaro

7. UHPLC in the Analyses of Isoflavones and Flavonoids 197
Sylwia Magiera and Irena Baranowska

8. UHPLC for Characterization of Protein Therapeutics 235
Jennifer C. Rea, Yajun Jennifer Wang, and Taylor Zhang

9. UHPLC/MS Analysis of Illicit Drugs 253
Guifeng Jiang, Jason R. Stenzel, Ray Chen, and Diab Elmashni

10. Ultra-High Performance Liquid Chromatography – Mass Spectrometry and Its Application 271
Zhili Xiong, Ying Deng, and Famei Li

Index 291

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