Bacteria from Fish and Other Aquatic Animals : A Practical Identification Manual

by Nicky B. Buller; John A. Plumb

• ISBN13: 9780851997384
• ISBN10: 0851997384
• Edition: 1st
• Format: Spiral Bound
• Copyright: 2004-06-17
• Publisher: STYLUS PUBLISHING LLC

Summary

*16 pages of colour plates to aid identification *Only published text available where all relevant material is referenced togther This manual enables the isolation and identification of bacteria that are found in aquatic animals, particularly fish. The emphasis is on bacteria from farmed aquatic animals, including fish,molluscs and crustacea, but some attention is also given to other marine and freshwater animals such as mammals and birds, both captive in zoos or in the wild, as well as aquarium fish.

Table of Contents

List of Tables and Figures	vii
Foreword	ix
J.A. Plumb
Acknowledgements	x
Introduction	xii
Aquatic Animal Species and Organism Relationship	1	(82)
Host Species, Bacteria and Disease	1	(1)
Bacterial Diseases	1	(36)
Bacteria and Relationship to Host	37	(38)
Taxonomy and Disease Status of Bacteria	75	(8)
Bacteriological Culture Techniques: Microscopy, Culture and Identification	83	(34)
Specimen Collection and Submission	84	(1)
Culture and Incubation	85	(1)
Examination of Culture Plates	85	(29)
Biochemical Identification Tests	114	(1)
Inoculation of Biochemical Identification Sets	115	(1)
API Identification Systems	116	(1)
Interpretation of Biochemical Identification Tests and Sets	117	(20)
Conventional Media: `Biochem Set'	117	(1)
Identification Tests and their Interpretation	117	(6)
Using the Biochemical Identification Tables	123	(1)
Interpretation and Identification of Genera and Species	123	(13)
Antisera Available	136	(1)
Biochemical Identification Tables	137	(85)
Results for Conventional Biochemical Tests -- `Biochem Set'	137	(1)
Results for API Kits	138	(84)
Technical Methods	222	(3)
Total Bacterial Count (TBC)	222	(2)
Microscopy	224	(1)
Storage of Isolates	224	(1)
Techniques for the Molecular Identification of Bacteria	225	(19)
Molecular Identification by PCR Using Specific Primers	225	(9)
PCR Protocols	234	(2)
Molecular Identification by 16S rDNA Sequencing	236	(6)
Fluorescence in Situ Hybridization (FISH)	242	(2)
Preparation of Media for Culture and Identification	244	(34)
General Isolation and Selective Media	244	(17)
Biochemical Test Media	261	(17)
Further Reading and Other Information Sources	278	(3)
Appendix: Common Name and Scientific Name of Aquatic Animals	281	(6)
Glossary of Terms	287	(5)
References	292	(37)
Index	329