BIOS Instant Notes in Molecular Biology

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  • Edition: 4th
  • Format: Nonspecific Binding
  • Copyright: 2012-06-06
  • Publisher: Taylor & Franci

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BIOS Instant Notes Molecular Biology, Fourth Edition, is the perfect text for undergraduates looking for a concise introduction to the subject, or a study guide to use before examinations. Each topic begins with a summary of essential facts-an ideal revision checklist-followed by a description of the subject that focuses on core information, with clear, simple diagrams that are easy for students to understand and recall in essays and exams. BIOS Instant Notes Molecular Biology, Fourth Edition, is fully up-to-date and covers: Information processing and macromolecules Properties of nucleic acids Prokaryotic and eukaryotic chromosome structure DNA replication DNA damage, repair and recombination Transcription in prokaryotes Regulation of transcription in prokaryotes Transcription in eukaryotes Regulation of transcription in eukaryotes RNA processing and RNPs The genetic code and tRNA Protein synthesis Bacteriophages and eukaryotic viruses Cell cycle and cancer Gene manipulation Cloning Vectors Gene libraries and screening Analysis and uses of cloned DNA Functional genomics and the new technologies

Table of Contents

Preface to the fourth editionp. v
Informational macromolecules
Information processing and molecular biologyp. 1
Nucleic acid structure and functionp. 4
Protein structure and functionp. 12
Macromolecular assembliesp. 22
Analysis of proteinsp. 26
Properties of nucleic acids
Chemical and physical properties of nucleic acidsp. 33
Spectroscopic and thermal properties of nucleic acidsp. 37
DNA supercoilingp. 40
Prokaryotic and eukaryotic chromosome structure
Prokaryotic chromosome structurep. 45
Chromatin structurep. 48
Eukaryotic chromosome structurep. 53
Genome complexityp. 59
DNA replication
DNA replication: an overviewp. 64
Bacterial DNA replicationp. 69
Eukaryotic DNA replicationp. 74
DNA damage, repair, and recombination
DNA damagep. 78
Mutagenesisp. 82
DNA repairp. 86
Recombination and transpositionp. 91
Transcription in bacteria
Basic principles of transcriptionp. 96
Escherichia coli RNA polymerasep. 99
The E. coli 70 promoterp. 102
Transcription initiation, elongation, and terminationp. 105
Regulation of transcription in bacteria
The lac operonp. 110
The trp operonp. 114
Transcriptional regulation by alternative a factors and RNAp. 119
Transcription in eukaryotes
The three RNA polymerases: characterization and functionp. 123
RNA Pol I genes: the ribosomal repeatp. 126
RNA Pol III genes: 5S and tRNA transcriptionp. 130
RNA Pol II genes: promoters and enhancersp. 134
General transcription factors and RNA Pol II initiationp. 137
Regulation of transcription in eukaryotes
Eukaryotic transcription factorsp. 141
Examples of transcriptional regulationp. 148
RNA processing and RNPs
rRNA processing and ribosomesp. 154
tRNA and other small RNA processingp. 160
mRNA processing, hnRNPs, and snRNPsp. 164
Alternative mRNA processingp. 171
The genetic code and tRNA
The genetic codep. 176
tRNA structure and functionp. 181
Protein synthesis
Aspects of protein synthesisp. 188
Mechanism of protein synthesisp. 192
Initiation in eukaryotesp. 199
Translational control and post-translational eventsp. 204
Bacteriophages and eukaryotic viruses
Introduction to virusesp. 210
Bacteriophagesp. 213
DNA virusesp. 218
RNA virusesp. 222
Cell cycle and cancer
The cell cyclep. 226
Oncogenesp. 231
Tumor suppressor genesp. 236
Apoptosisp. 240
Gene manipulation
DNA cloning: an overviewp. 244
Preparation of plasmid DNAp. 249
Restriction enzymes and electrophoresisp. 253
Ligation, transformation, and analysis of recombinantsp. 258
Cloning vectors
Design of plasmid vectorsp. 265
Bacteriophages, cosmids, YACs, and BACsp. 270
Eukaryotic vectorsp. 278
Gene libraries and screening
Genomic librariesp. 283
cDNA librariesp. 286
Screening proceduresp. 290
Analysis and uses of cloned DNA
Characterization of clonesp. 293
Nucleic acid sequencingp. 297
Polymerase chain reactionp. 303
Analysis of cloned genesp. 309
Mutagenesis of cloned genesp. 313
Functional genomics and the new technologies
Introduction to the 'omicsp. 317
Global gene expression analysisp. 321
Proteomicsp. 328
Cell and molecular imagingp. 333
Transgenics and stem cell technologyp. 337
Bioinformaticsp. 341
Systems and synthetic biologyp. 350
Further readingp. 356
Abbreviationsp. 365
Indexp. 368
Table of Contents provided by Ingram. All Rights Reserved.

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