Capillary Electrochromatography

by Bartle, Keith D.; Myers, Pete

• ISBN13: 9780854045303
• ISBN10: 0854045309
• Format: Hardcover
• Copyright: 2000-03-01
• Publisher: Royal Society of Chemistry

Summary

Capillary electrochromatography (CEC) is a new and exciting hybrid separation technique that seeks to exploit the combined advantages of both capillary electrophoresis (high efficiencies) and HPLC (mobile and stationary phase selectivity). It is a technique with tremendous potential, especially in the pharmaceutical and biomedical fields. This is the first book to be devoted to the topic and presents reviews by the world leaders in the field on the theory and development of the technique and current and potential future applications. "Capillary Electrochromatography" provides an excellent introduction to the field for graduates and professionals in industry and academia with an interest in separation science.[part of the RSC Chromatography Monographs series]

Table of Contents

An Introduction to Capillary Electrochromatography	1	(22)
Keith D. Bartle
Maria G. Cikalo
Mark M. Robson
What Is Capillary Electrochromatography?	1	(1)
History of CEC	2	(3)
Electroosmosis	5	(2)
Classification of CEC	7	(2)
Band-broadening in CEC	9	(3)
Effect of Operating Variables in CEC	12	(5)
Application Areas of CEC	17	(6)
References	20	(3)
The Capillary Electrochromatograph	23	(10)
Norman W. Smith
Introduction	23	(1)
Modification of Standard CZE Equipment	23	(4)
Sample Injection Using Modified CZE Equipment	27	(1)
Modification of CZE Equipment with Autosampler Capability	28	(1)
Commercial CEC Instruments	29	(1)
Future Instrument Designs and Requirements	30	(3)
References	31	(2)
Supports and Stationary Phases for Capillary Electrochromatography	33	(9)
Peter Myers
Introduction	33	(1)
Particle Size Distributions	34	(1)
The Silica Surface	35	(1)
Bonding Technology	36	(1)
Effect of pH on the Silanols	37	(1)
Analyte Focusing	38	(2)
Anion Exchange Materials	40	(1)
Continuous Bed Columns	40	(1)
Conclusions	41	(1)
References	41	(1)
Electroosmosis in Complex Media: Bulk Transport in CEC	42	(22)
Vincent T. Remcho
Patrick T. Vallano
Introduction	42	(1)
Electroosmotic Flow	43	(4)
Electroosmosis in Packed Beds	47	(13)
Electroosmosis in Porous Monoliths	60	(2)
Conclusion	62	(2)
References	62	(2)
Capillary Electrochromatography with Open Tubular Columns (OTCEC)	64	(23)
Monika M. Dittmann
Gerard P. Rozing
Introduction	64	(1)
Chromatographic Effects in CZE	65	(3)
Theory of Dispersion and Achievable Efficiency in OTLC and OTCEC	68	(5)
Improving the Phase Ratio for OTCEC	73	(10)
Conclusions	83	(4)
References	84	(3)
Capillary Electrochromatography/Mass Spectrometry	87	(20)
G. A. Lord
D. B. Gordon
Introduction	87	(1)
Mass Spectrometry	87	(6)
Capillary Electrochromatography/Mass Spectrometry Coupling	93	(7)
Practical Aspects	100	(4)
Future Prospects	104	(3)
References	105	(2)
Pharmaceutical Applications of Capillary Electrochromatography	107	(18)
Melvin R. Euerby
Nicola C. Gillott
Introduction	107	(1)
Scope and Applicability of CEC	108	(3)
Pharmaceutical Applications of CEC	111	(7)
Issues Associated with CEC in Pharmaceutical Analysis	118	(3)
The Future of CEC in the Pharmaceutical Industry	121	(4)
References	122	(3)
Capillary Electrochromatography in Natural Product Research	125	(21)
An Dermaux
Pat Sandra
Introduction	125	(1)
Experimental	125	(1)
Triglycerides	126	(9)
Fatty Acids and Their Derivatives	135	(7)
CEC Analysis of Other `Hydrophobic' Samples	142	(2)
Conclusion	144	(2)
References	144	(2)
Subject Index	146