DNA Sequencing Protocols

by Graham, Colin A.; Hill, Alison J. M.

• ISBN13: 9780896037212
• ISBN10: 0896037215
• Edition: 2nd
• Format: Paperback
• Copyright: 2001-01-01
• Publisher: Humana Pr Inc

Summary

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Table of Contents

Preface	v
Contributors	ix
Introduction to DNA Sequencing	1	(12)
Colin A. Graham
Alison J. M. Hill
The Universal Primers and the Shotgun DNA Sequencing Method	13	(20)
Joachim Messing
M13 Sequencing	33	(6)
Qingzhong Yu
Primer Design and Primer-Directed Sequencing	39	(14)
Ulrike Gerischer
Peter Durre
Direct Sequencing of DNA Produced in a Polymerase Chain Reaction	53	(10)
Ulrike Gerischer
Solid Phase Fluorescent Sequencing of the CFTR Gene	63	(26)
Harry Cuppens
Jean-Jacques Cassiman
Shotgun DNA Sequencing	89	(12)
Alan T. Bankier
Cycle Sequencing	101	(12)
Robert W. Blakesley
Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers	113	(6)
Johannes Zschocke
Georg F. Hoffmann
Automated Fluorescent DNA Sequencing on the ABI PRISM 377	119	(34)
Jonathan R. E. MacBeath
Suzanna S. Harvey
Nicola J. Oldroyd
Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer	153	(18)
David Watts
Jonathan R. E. MacBeath
Fluorescent Sequencing Protocols for the ALF	171	(22)
Shirley McQuaid
Fluorescent Sequencing for Heterozygote Mutation Detection	193	(22)
Colin A. Graham
Alison J. M. Hill
Sequence Databases and the Internet	215	(10)
Anne E. Hughes
DNA Sequencing by Capillary Array Electrophoresis	225	(16)
Norman J. Dovichi
JianZhong Zhang
Index	241