9780879695866

Drosophila Protocols

by
  • ISBN13:

    9780879695866

  • ISBN10:

    0879695862

  • Edition: Lab
  • Format: Hardcover
  • Copyright: 2000-03-01
  • Publisher: Cold Spring Harbor Laboratory Pr

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

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Supplemental Materials

What is included with this book?

Summary

Univ. of California, Santa Cruz. Describes more than 30 procedures used for molecular, biochemical biochemical and cellular studies on Drosophilia. Features include: scope, depth, format, and added value. Chapters include figures, tables, illustrations, and examples. For novice and experienced researchers. NOTE: Softcover edition, ISBN 0879695854, will not be published per publ.

Table of Contents

Preface ix
Abbreviations xi
CHROMOSOMES
Preparation and Analysis of Drosophila Mitotic Chromosomes
3(22)
S. Pimpinelli
S. Bonaccorsi
L. Fanti
M. Gatti
In Situ Hybridization to Somatic Chromosomes
25(32)
A.F. Dernburg
BrdU Labeling of Chromosomes
57(10)
A.W. Shermoen
Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy
67(20)
H.J.G. Matthies
M. Clarkson
R.B. Saint
R. Namba
R.S. Hawley
Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster
87(24)
S. Bonaccorsi
M.G. Giansanti. G. Cenci
M. Gatti
Preparation and Analysis of Polytene Chromosomes
111(8)
J.A. Kennison
In Situ Hybridization to Polytene Chromosomes
119(12)
M.-L. Pardue
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
131(10)
R. Paro
CELL BIOLOGY
Fluorescent Analysis of Drosophila Embryos
141(18)
W.F. Rothwell
W. Sullivan
Imaginal Discs
159(16)
S.S. Blair
The Neuromuscular Junction
175(26)
H.J. Bellen
V. Budnik
Histological Techniques for the Drosophila Eye. Part I: Larva and Pupa
201(28)
T. Wolff
Histological Techniques for the Drosophila Eye. Part II: Adult
229(16)
T. Wolff
Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy
245(28)
K.L. McDonald
D.J. Sharp
W. Rickoll
Electrophysiological Approaches to the Neuromusculature
273(24)
K.S. Broadie
Functional Assays of the Peripheral and Central Nervous Systems
297(16)
K.S. Broadie
GFP and Other Reporters
313(32)
T. Hazelrigg
Quantitative Microinjection of Drosophila Embryos
345(16)
D.P. Kiehart
J.M. Crawford
R.A. Montague
Targeted Disruption of Gene Function in Drosophila by RNA Interference
361(12)
L. Misquitta
B.M. Paterson
Drosophila Cell Culture and Transformation
373(16)
L. Cherbas
P. Cherbas
Generating Antibodies against Drosophila Proteins
389(24)
I. Rebay
R.G. Fehon
Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation
413(16)
J. Minden
R. Namba
S. Cambridge
MOLECULAR BIOLOGY
Recovery of DNA Sequences Flanking P-element Insertions: Inverse PCR and Plasmid Rescue
429(10)
A.M. Huang
E.J. Rehm
G.M. Rubin
GAL4-mediated Ectopic Gene Expression in Drosophila
439(10)
P. van Roessel
A.H. Brand
Functional Cell Ablation
449(30)
S.T. Sweeney
A. Hidalgo
J.S. deBelle
H. Keshishian
GENOMICS
Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones
479(8)
E.R. Lozovskaya
Drosophila Microarrays: From Arrayer Construction to Hybridization
487(22)
K.P. White
K.C. Burtis
Using Drosophila Genome Databases
509(16)
S. Misra
M.A. Crosby
R.A. Drysdale
Preparation and Analysis of High-molecular-weight DNA in Drosophila
525(16)
G.H. Karpen
BIOCHEMISTRY
Culturing Large Populations of Drosophila for Protein Biochemistry
541(12)
J.C. Sisson
Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis
553(10)
M.J. Pazin
Preparation of Membrane Proteins from Drosophila Embryos
563(8)
C.X. Zhang
T.-S. Hsieh
Preparing Cytoplasmic Extracts from Drosophila Embryos
571(6)
M. Moritz
Immunoblotting of Proteins from Single Drosophila Embryos
577(8)
T.T. Su
THE ORGANISM
Laboratory Culture of Drosophila
585(16)
M. Ashburner
J. Roote
Preparation of Larval and Adult Cuticles for Light Microscopy
601(16)
D.L. Stern
E. Sucena
Exposing Drosophila to Neuroactive Drugs
617(68)
J. Hirsh
APPENDICES
1 1989 Table of Contents from Drosophila: A Laboratory Manual
625(10)
2 Anatomical Drawings of Drosophila
635(20)
3 Solutions and Reagents
655(6)
4 Cautions
661(18)
5 Suppliers
679(2)
6 Trademarks
681(4)
Index 685

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