Enzyme Technologies Metagenomics, Evolution, Biocatalysis and Biosynthesis

by Yeh, Wu-Kuang; Yang, Hsiu-Chiung; McCarthy, James R.

• ISBN13: 9780470286241
• ISBN10: 0470286245
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-09-28
• Publisher: Wiley

Summary

Enzyme Technologies in Drug Discovery highlights how, what, and where enzymes have become critical in pharmaceutical and biotechnology research. This book provides in-depth reviews of recent developments in biosynthesis, biocatalysis, and the chemical biology of enzymes. This text discusses enzymatic assays, including emerging assay technologies for key enzyme classes in pharmaceutical research. In addition to new developments in proteomics, this book includes two emerging technologies in life sciences, metabolomics and preclinomics. This volume reviews important progress on the chemical biology of enzymes in the post-genomic era.

Author Biography

Wu-Kuang Yeh, PhD, is Executive Consultant and was director of research and principal investigator at PreClinOmics, Inc., from 2004-2010. He was a principal research scientist at Eli Lilly and Company from 1982-2004. He is the author or coauthor of over 115 abstracts and publications, coeditor of one book, and the holder of six U.S. patents. Hsiu-Chiung Yang, PhD, is a Research Advisor at Eli Lilly and Company. She was a principal research scientist at Eli Lilly and Company from 1998-2007 and is the author or coauthor of over forty abstracts and publications, as well as co-inventor of one patent. James R. McCarthy, PhD, is the author, coauthor, or coeditor of over 125 scientific publications and holder of over forty U.S. patents. He is on the editorial advisory board of the Journal of Medicinal Chemistry and is a past chair of the division of Medicinal Chemistry of the American Chemical Society. He also serves as a Senior Scientific Advisor for the Huntington Disease Research Foundation.

Table of Contents

Contributors	p. vii
Preface	p. ix
New Approaches to Finding and Modifying Enzymes	p. 1
Functional Metagenomics as a Technique for the Discovery of Novel Enzymes and Natural Products	p. 3
Directed Enzyme and Pathway Evolution	p. 41
Combining Natural Biodiversity and Molecular-Directed Evolution to Develop New Industrial Biocatalysts and Drugs	p. 77
Principles of Enzyme Optimization for the Rapid Creation of Industrial Biocatalysts	p. 99
Biocatalytic Applications	p. 125
Enzyme Catalysis in the Synthesis of Active Pharmaceutical Ingredients	p. 127
Enzymatic Processes for the Production of Pharmaceutical Intermediates	p. 185
Novel Developments Employing Redox Enzymes: Old Enzymes in New Clothes	p. 199
Biosynthetic Applications	p. 251
Drug Discovery and Development by Combinatorial Biosynthesis	p. 253
Reprogramming Daptomycin and A54145 Biosynthesis to Produce Novel Lipopeptide Antibiotics	p. 285
Pathway and Enzyme Engineering and Applications for Glycodiversification	p. 309
Index	p. 363
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