Explorations in Microbiology A Discovery-Based Approach

by Hudson, Barbara K.; Sherwood, Linda M.

• ISBN13: 9780135335895
• ISBN10: 0135335892
• Edition: 1st
• Format: Paperback
• Copyright: 1997-02-11
• Publisher: Pearson

Summary

This collection of 43 laboratory exercises not only introduces basic techniques in microbiology, but reinforces major concepts and themes in microbiology as well.Promotes critical thinking through open-ended, discovery-based exercises, application/integration exercises, and exercises that require students to work in groups.

Table of Contents

Laboratory Safety Procedures.

I. GETTING STARTED.

1. Growing Microbes on Solid Surfaces.
2. Wet Mounts and Microscopy—A Microbiology Legacy.
3. Streaking Plates for Isolated Colonies.
4. Killing Microbes.
5. “Seeing More” With Phase Microscopy.
6. Important Microbiological Stains.
7. Modern Staining Procedures.
8. Some Like It Hot!
9. Microbe Safari.

II. THE MICROBES.

10. The Classification Game.
11. Protozoa — Friend and Foe.
12. Algae.
13. A Fungus Among Us!
14. Identifying Structural Features of Bacteria Using Special Stains.
15. Identifying Bacteria Using Metabolic Characteristics.
16. New Directions in Classification and Identification.
17. Growing Viruses.
18. What Kind of Microbe Is It?

III. MICROBIAL GROWTH AND REPRODUCTION.

19. The Quest for Energy.
20. First There Was One, Then There Were Two.
21. Eucaryotic Reproduction.
22. “Sex” Lives of Bacteria.

IV. MICROBIAL GENETICS.

23. The Search for Mutants.
24. Isolation of Plasmids.
25. At the Cutting Edge.
26. Transformation of Escherichia coli With Plasmid DNA.

V. MICROBIAL ECOLOGY.

27. Tower of Microbes.
28. Enriching for Aerobic, Nitrogen-Fixing Bacteria.
29. Water, Water, Everywhere.
30. Growing Microbes on Rocks.
31. Humans as Habitats.

VI. MEDICAL MICROBIOLOGY.

32. Sputum, Pus and Other Slimy Stuff: Pathogenic Gram-Positive Cocci of the Respiratory Tract, Wounds, and Abscesses.
33. Pathogens of the Urinary Tract and Gastrointestinal Tract: The Enterobacteriaceae.
34. Sleuthing for a Parasite.
35. Passing the Germ.
36. Who (or What) is to Blame?
37. Too Much Resistance.
38. Name That Pathogen.

VII. HOST DEFENSES.

39. Phagocytosis.
40. Antigen-Antibody Reactions.
41. Measuring Antibody Titer by ELISA.
42. Monoclonal Antibodies.

VIII. BIOTECHNOLOGY.

43. A Day of Feasting Courtesy of the Microbes.
44. Biofilms and Space Flight.
45. The Problem with Packing Materials.

APPENDICES.

Appendix A: How Lenses, Magnifiers, and Microscopes Work.
Appendix B: Writing Laboratory Reports.