Gene Cloning and DNA Analysis: An Introduction, 6th Edition

by Terry Brown (University of Manchester )

• ISBN13: 9781444318616
• ISBN10: 1444318616
• Format: eBook
• Copyright: 2010-04-01
• Publisher: Wiley-Blackwell
• More Book Details
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Summary

Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." - Journal of Heredity, 2007 (on the previous edition)

Table of Contents

Preface to the Sixth Edition

The Basic Principles of Gene Cloning and DNA Analysis

Why Gene Cloning and DNA Analysis are Important

The early development of genetics

The advent of gene cloning and the polymerase chain reaction

What is gene cloning?

What is PCR?

Why gene cloning and PCR are so important

How to find your way through this book

Vectors for Gene Cloning: Plasmids and Bacteriophages

Plasmids

Bacteriophages

Purification of DNA from Living Cells

Preparation of total cell DNA

Preparation of plasmid DNA

Preparation of bacteriophage DNA

Manipulation of Purified DNA

The range of DNA manipulative enzymes

Enzymes for cutting DNA - restriction endonucleases

Ligation - joining DNA molecules together

Introduction of DNA into Living Cells

Transformation - the uptake of DNA by bacterial cells

Identification of recombinants

Introduction of phage DNA into bacterial cells

Identification of recombinant phages

Introduction of DNA into non-bacterial cells

Cloning Vectors for E. coli

Cloning vectors based on E. coli plasmids

Cloning vectors based on M13 bacteriophage

Cloning vectors based on l bacteriophage

¿ and other high capacity vectors enable genomic libraries to be constructed

Vectors for other bacteria

Cloning Vectors for Eukaryotes

Vectors for yeast and other fungi

Cloning vectors for higher plants

Cloning vectors for animals

How to Obtain a Clone of a Specific Gene

The problem of selection

Direct selection

Identification of a clone from a gene library

Methods for clone identification

The Polymerase Chain Reaction

The polymerase chain reaction in outline

PCR in more detail

After the PCR: studying PCR products

Real time PCR enables the amount of starting material to be quantified

The Applications of Gene Cloning and DNA Analysis in Research

Sequencing Genes and Genomes

The methodology for DNA sequencing

How to sequence a genome

Studying Gene Expression and Function

Studying the RNA transcript of a gene

Studying the regulation of gene expression

Identifying and studying the translation product of a cloned gene

Studying Genomes

Genome annotation

Studies of the transcriptome and proteome

The Applications of Gene Cloning and DNA Analysis in Biotechnology

Production of Protein from Cloned Genes

Special vectors for expression of foreign genes in E. coli

General problems with the production of recombinant protein in E. coli

Production of recombinant protein by eukaryotic cells

Gene Cloning and DNA Analysis in Medicine

Production of recombinant pharmaceuticals

Identification of genes responsible for human diseases

Gene therapy

Gene Cloning and DNA Analysis in Agriculture

The gene addition approach to plant genetic engineering

Gene subtraction

Problems with genetically modified plants

Gene Cloning and DNA Analysis in Forensic Science and Archaeology

DNA analysis in the identification of crime suspects

Studying kinship by DNA profiling

Sex identification by DNA analysis

Archaeogenetics - using DNA to study human prehistory

Glossary

Index

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