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9780470033562

Human Embryonic Stem Cells The Practical Handbook

by ; ;
  • ISBN13:

    9780470033562

  • ISBN10:

    0470033568

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 7/23/2007
  • Publisher: WILEY

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Summary

With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches.Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo.The novel and useful focus of this book sets it apart from other available books:Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cellsCentred solely on practical protocols for human (not mouse) embryonic stem cell researchIncludes extensive troubleshooting sectionsAddresses the different proclivities and behaviours of individual human embryonic cell linesContains techniques currently known only to a small number of specialised laboratories worldwideThis handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases.

Author Biography

Stephen Sullivan. Harvard University, Cambridge, MA, USA

Kevin Eggan. Harvard University, Cambridge, MA, USA

Chad A. Cowan. Harvard University, Cambridge, MA, USA

Table of Contents

Foreword
Preface
Obtaining and culturing human embryonic stem cells
Organization and good aseptic technique in the human embryonic stem cell laboratory
Sourcing human embryonic stem cell lines
Human embryo culture for human embryonic stem cell derivation
Derivation of human embryonic stem cell lines
Standard culture of human embryonic stem cells
Culture of human embryonic stem cells in chemically defined conditions
Characterization of undifferentiated embryonic stem cells
Phenotypic analysis of human embryonic stem cells
Genetic and epigenetic analysis of human embryonic stem cells
Manipulation of human embryonic stem cells
In vivo differentiation of human embryonic stem cells
In vitro differentiation of human embryonic stem cells
Differentiation of human embryonic stem cells into extraembryonic cell types
Directed differentiation of human embryonic stem cells into
Early endoderm cells
Hepatic cells
Pancreatic cells
Directed differentiation of human embryonic stem cells into
Cardiomyocytes Christine
Endothelial cells
Osteogenic cells
Hematopoietic in vivo repopulating cells
Lymphocytes
Myeloid cells
Directed differentiation of human embryonic stem cells into
Forebrain neurons
Dopaminergic neuron
Spinal motor neurons
Gene targeting: knock out and knock in by homologous recombination
RNA interference in human embryonic stem cells
Generation of gene reporters using bacterial artificial chromosome recombineering
Table of Contents provided by Publisher. All Rights Reserved.

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