Laboraatory Fundamentals of Microbiology

by Alcamo, I. Edward, Phd

• ISBN13: 9780763712358
• ISBN10: 0763712353
• Edition: 6th
• Format: Paperback
• Copyright: 2000-08-01
• Publisher: Jones & Bartlett Pub

Summary

State Univ. of New York, Farmingdale. Textbook and workbook for introductory laboratory courses in microbiology. Designed for use with the author's Fundamentals of Microbiology, 6th ed., c2001. Thirty lab exercises provide basic training in handling microorganisms while exploring microbial properties and uses. Wire-spiral binding with three-hole punched, perforated pages.

Table of Contents

Preface	vii
An Important Message About Safety	ix
The Compound Microscope	1	(8)
Preparation of a Bacterial Smear and the Simple Stain Technique	9	(8)
Preparation of a Bacterial Smear	10	(2)
The Simple Stain Technique	12	(5)
The Negative Stain Technique	17	(6)
The Gram Stain Technique	23	(8)
Bacterial Structures	31	(10)
Spore Stain Technique	31	(2)
Capsule Stain Technique	33	(2)
Bacterial Motility	35	(6)
Pure Culture Techniques	41	(10)
Streak Plate Technique	41	(3)
Pour Plate Technique	44	(7)
Bacterial Culture Characteristics	51	(6)
Biochemical Characteristics of Bacteria	57	(16)
Fermentation of Carbohydrates	57	(1)
Digestion of Starch	58	(2)
Catalase Production	60	(1)
DNA Digestion	61	(1)
Hydrogen Sulfide Production	62	(1)
Indole Production	63	(1)
Urea Digestion	64	(1)
Lipid Digestion	65	(1)
Casein Digestion	66	(7)
Identification of a Bacterial Unknown	73	(8)
Induced and Spontaneous Mutations in Bacteria	81	(8)
Mutagenic Effect of Ultraviolet Light	81	(2)
Gradient Plate Technique	83	(6)
Fungi, Protozoa, and Multicellular Parasites	89	(12)
Fungi (Molds and Yeast)	89	(5)
Protozoa and Multicellular Parasites	94	(7)
Viruses	101	(10)
Constructing a Virus	101	(1)
The Effect of Bacteriophages on Bacteria	102	(2)
Plaque Formation	104	(1)
Identification of Bacteriophages from Sewage	105	(6)
Agglutination Techniques	111	(10)
Slide Agglutination	111	(1)
Tube Agglutination	112	(2)
Hemagglutination	114	(2)
Blood Smear	116	(5)
A Simulated Epidemic	121	(8)
Microbial Transmission via Formites	121	(2)
A Microbial Hunt	123	(2)
Transmission of Microorganisms Through Toilet Paper	125	(4)
The Genus Mycobacterium	129	(8)
Colonial and Cellular Morphology	129	(1)
Acid-Fast Stain Technique	130	(2)
Cold Acid-Fast Stain Technique	132	(5)
The Genus Streptococcus	137	(6)
Streptococci from the Upper Respiratory Tract	137	(2)
Streptococci from the Oral Cavity	139	(4)
The Genus Neisseria	143	(4)
The Genus Staphylococcus	147	(6)
Isolation of Staphylococci	147	(1)
Coagulase Activity	148	(1)
Other Properties of Staphylococci	148	(5)
The Enteric Bacteria	153	(12)
Isolation of Enteric Bacteria	153	(1)
Differentiation of Enteric Bacteria on TSI Agar	154	(2)
The IMViC Series	156	(1)
Rapid Identification of Enteric Bacteria	157	(8)
The Genus Bacillus	165	(4)
The Genus Clostridium	169	(6)
The Genus Lactobacillus	175	(6)
Isolation of Lactobacilli	175	(1)
Caries Susceptibility Test	176	(5)
The Effect of Physical Agents on Bacteria	181	(12)
Heat	181	(2)
Ultraviolet Light	183	(2)
Drying	185	(1)
Other Physical Agents	186	(7)
The Effect of Chemical Agents on Bacteria	193	(14)
Antiseptics and Disinfectants	193	(2)
Metals	195	(1)
Other Chemical Substances	196	(1)
Effect of Lysozyme on Bacteria	196	(2)
Disinfection of Drinking Water	198	(9)
Evaluation of Disinfectants and Antiseptics	207	(10)
Effectiveness of Disinfectants on Inanimate Objects	207	(1)
Effectiveness of Disinfectants and Hand Washing on the Skin Surface	208	(1)
Mouthwashes and Oral Bacteria	209	(1)
The Phenol Coefficient	210	(7)
The Effect of Antibiotics on Bacteria	217	(10)
Microbiology of Foods	227	(14)
Food Preservation with Salt and Garlic	227	(1)
Standard Plate Count of Food Products	228	(3)
Fermentation of Sauerkraut	231	(1)
Fermentation of Wine and Beer	232	(9)
Microbiology of Milk and Dairy Products	241	(14)
Standard Plate Count of Milk	241	(2)
Coliform Plate Count of Milk	243	(1)
Methylene Blue Reduction Test	244	(1)
Preparation of Cheese	245	(1)
Preparation of Yogurt	246	(1)
The Natural Flora of Milk	247	(8)
Microbiology of Water	255	(12)
Presumptive Test by the MPN Method	255	(1)
Confirmed Test	256	(2)
Completed Test	258	(1)
Membrane Filter Technique	259	(1)
Preparation of a Biofilm by Two Methods	260	(7)
Microbiology of Soil	267	(18)
Isolation of Rhizobium from Legume Roots	267	(1)
Ammonification by Soil Microorganisms	268	(1)
Isolation of Streptomyces from Soil	269	(1)
Antibiotic Production by Streptomyces	269	(2)
Plate Count of Soil Bacteria	271	(1)
Microbial Ecology in the Soil-The Winogradsky Column	271	(2)
Recycling of Carbon in Food	273	(2)
Recycling of Carbon in Cloth	275	(1)
Recycling of Carbon in Leaves	276	(9)
Appendix A Principles of Laboratory Safety	285	(4)
Appendix B Investigations in Microbiology	289	(2)
Appendix C To the Instructor: A Laboratory Program in Microbiology	291	(7)
Appendix D Laboratory Media, Cultures, and Special Materials	298	(2)
Appendix E Preparation of Stains and Diagnostic Reagents	300	(2)
Appendix F Using Enterotube II	302	(3)
Index	305