Laboratory Applications in Microbiology

by Chess, Barry

• ISBN13: 9780073402376
• ISBN10: 0073402370
• Edition: 2nd
• Format: Spiral Bound
• Copyright: 2011-02-15
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

Laboratory Applications in Microbiology: A Case Study Approachnow with an included photo atlas including more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Table of Contents

Case Study Exercise 1 Safety Considerations in the Microbiology Laboratory

Case Study Exercise 2 Microscopy and Measurement of Microscopic Specimens

Case Study Exercise 3 Identification and Classification of Algae

Case Study Exercise 4 Survey of Medically Important Protozoa

Case Study Exercise 5 Identification and Classification of Fungi

Case Study Exercise 6 Ubiquity of Microorganisms

Case Study Exercise 7 Aseptic Techniques

Case Study Exercise 8 Pure Culture Techniques

Case Study Exercise 9 Simple Staining, Negative Staining, and Gram Staining

Case Study Exercise 10 Capsular Staining

Case Study Exercise 11 Acid-Fast and Endospore Staining

Case Study Exercise 12 Viable Plate Count

Case Study Exercise 13 Cultivation of Anaerobes

Case Study Exercise 14 Temperature Effects on Bacterial Growth and Survival

Case Study Exercise 15 pH and Microbial Growth

Case Study Exercise 16 Effects of Osmotic Pressure on Bacterial Growth

Case Study Exercise 17 Lethal Effects of Ultraviolet Light

Case Study Exercise 18 Evaluation of Disinfectants: Use-Dilution Method

Case Study Exercise 19 Effectiveness of Hand Scrubbing

Case Study Exercise 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods

Case Study Exercise 21 Phage Typing of Bacteria

Case Study Exercise 22 Simulated Epidemic

Case Study Exercise 23 Morbidity and Mortality Weekly Report

Case Study Exercise 24 Bacterial Transformation

Case Study Exercise 25 The Ames Test

Case Study Exercise 26 DNA Extraction from Bacterial Cells

Case Study Exercise 27 DNA Profiling

Case Study Exercise 28 Measures of Water Quality: Most Probable Number Procedure

Case Study Exercise 29 Measures of Water Quality: Membrane Filtration Method

Case Study Exercise 30 Measures of Milk Quality: Methylene Blue Reductase Test

Case Study Exercise 31 Bacterial Counts of Food

Case Study Exercise 32 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae

Case Study Exercise 33 Isolation and Identification of Staphylococci

Case Study Exercise 34 Isolation and Identification of Streptococci

Case Study Exercise 35 Blood Typing

Case Study Exercise 36 Differential White Blood Cell Count

Case Study Exercise 37 Slide Agglutination

Case Study Exercise 38 Enzyme Linked Immunosorbent Assay (ELISA)

Case Study Exercise 39 Identification of Bacterial Unknowns

Exercise 40 Colony Morphology

Exercise 41 Growth in Solid and Liquid Media

Exercise 42 Simple Stain

Exercise 43 Negative Stain

Exercise 44 Capsule Stain

Exercise 45 Gram Stain

Exercise 46 Endospore Stain

Exercise 47 Acid-Fast Stain

Exercise 48 Motility Methods: Wet Mount and Hanging Drop

Exercise 49 Flagella Stain

Exercise 50 Streak-Plate Isolation

Exercise 51 Loop Dilution

Exercise 52 Spread-Plate

Exercise 53 Fluid Thioglycollate Medium

Exercise 54 Mannitol Salt Agar

Exercise 55 MacConkey Agar

Exercise 56 Desoxycholate Agar

Exercise 57 Endo Agar

Exercise 58 Eosin Methylene Blue Agar

Exercise 59 Hektoen Enteric Agar

Exercise 60 Xylose Lysine Desoxycholate Agar

Exercise 61 Blood Agar

Exercise 62 Motility Media

Exercise 63 SIM Medium

Exercise 64 Kligler’s Iron Agar

Exercise 65 Triple Sugar Iron Agar

Exercise 66 Lysine Iron Agar

Exercise 67 Litmus Milk

Exercise 68 Oxidation-Fermentation Test

Exercise 69 Phenol Red Broth

Exercise 70 Purple Broth

Exercise 71 Methyl Red and Voges-Proskauer Tests

Exercise 72 Catalase Test

Exercise 73 Oxidase Test

Exercise 74 Nitrate Reduction Test

Exercise 75 Coagulase Test

Exercise 76 Citrate Test

Exercise 77 Malonate Test

Exercise 78 Decarboxylation Test

Exercise 79 Phenylalanine Deaminase Test

Exercise 80 Bile Esculin Test

Exercise 81 Starch Hydrolysis

Exercise 82 ONPG Test

Exercise 83 Urease Test

Exercise 84 Casease Test

Exercise 85 Gelatinase Test

Exercise 86 DNase Test

Exercise 87 Lipase Test

Exercise 88 CAMP Test

Exercise 89 PYR Test

Exercise 90 API 20E System

Exercise 91 Enterotube II System

Exercise 92 Antibiotic Disk Sensitivity Tests

Exercise 93 β-Lactmase Test

Exercise 94 Viable Plate Count

Exercise 95 Direct Cell Count

Appendix A Spectrophotometric Determination of Bacterial Growth: Use of the Spectrophotometer

Appendix B Use of Pipettes in the Laboratory

Appendix C Preparation of Culture Media