Laboratory Experiments in Microbiology

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  • Edition: 9th
  • Format: Spiral Bound
  • Copyright: 2009-01-06
  • Publisher: Benjamin Cummings
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Key Benefit: Containing 57 thoroughly class-tested exercises, this manual provides basic microbiology techniques with applications for undergraduate readers in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Ninth Edition features a new, four-color design and a dramatically new art program. Many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style, and detailed, colorful photomicrographs that were once grouped together in a color insert are now integrated throughout the exercises. Experiments have been refined throughout, and a new exercise on parasitic helminths readers with valuable practice in microscopic examination and observation.

Key Topics: Use and Care of the Microscope, Examination of Living Microorganisms, Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining,

Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria, Carbohydrate Catabolism, Fermentation, Protein Catabolism, Part 1, Protein Catabolism, Part 2, Respiration,

Unknown Identification and Bergey’s Manual, Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms, Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing, Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting,

Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens, Fungi: Yeasts and Molds, Phototrophs: Algae and Cyanobacteria 35. Protozoa, Parasitic Helminths, Isolation and Titration of Bacteriophages, Plant Viruses, Epidemiology, Koch’s Postulate, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique, Bacteria of the Skin, Bacteria of the Respiratory Tract,Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Genitourinary Tract, Rapid Identification Methods, Identification of an Unknown from a Clinical Sample, Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique,

Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles,

Microbes in Soil: Bioremediaton

Market: Intended for those interested in learning the basics of microbiology

Author Biography

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 31 years and previously taught at Mankato State University. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several abroad semester and interim programs. His research focuses on the immune response to virally induced cancer in various animal models.


Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 38 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

Table of Contents

Use and Care of the Microscope
Examination of Living Microorganisms
Handling Bacteria
Microbes in the Environment
Transfer of Bacteria: Aseptic Techniques
Staining Methods
Preparation of Smears and Simple Staining
Negative Staining
Gram Staining
Acid-fast Staining
Structural Stains (Endospore, Capsule, Flagella)
Morphologic Unknown
Cultivation Of Bacteria
Isolation of Bacteria by Dilution Technique
Special Media for Isolating Bacteria
Microbial Metabolism
Carbohydrate Catabolism
Protein Catabolism, Part 1
Protein Catabolism, Part 2
Unknown Identification and Bergey's Manual
Microbial Growth
Oxygen and the Growth of Bacteria
Determination of a Bacterial Growth Curve: The Role of Temperature
Control Of Microbial Growth
Physical Methods of Control: Heat
Physical Methods of Control: Ultraviolet Radiation
Chemical Methods of Control: Disinfectants and Antiseptics
Chemical Methods of Control: Antimicrobial Drugs
Effectiveness of Hand Scrubbing
Microbial Genetics
Regulation of Gene Expression
Isolation of Bacterial Mutants
Transformation of Bacteria
DNA Fingerprinting
Genetic Engineering
Ames Test for Detecting Possible Chemical Carcinogens
The Microbial World
Fungi: Yeasts and Molds
Phototrophs: Algae and Cyanobacteria 35. Protozoa
Parasitic Helminths
Isolation and Titration of Bacteriophages
Plant Viruses
Interaction Of Microbe And Host
Koch's Postulate
Nonspecific Resistance
Blood Group Determination: Slide Agglutination
Agglutination Reactions: Microtiter Agglutination
ELISA Technique
Microorganisms And Disease
Bacteria of the Skin
Bacteria of the Respiratory Tract
Bacteria of the Mouth
Bacteria of the Gastrointestinal Tract
Bacteria of the Genitourinary Tract
Rapid Identification Methods
Identification of an Unknown from a Clinical Sample
Microbiology And The Environment
Microbes in Water: Multiple-Tube Technique
Microbes in Water: Membrane Filter Technique
Microbes in Food: Contamination
Microbes Used in the Production of Foods
Microbes in Soil: The Nitrogen and Sulfur Cycles
Microbes in Soil: Bioremediaton
Dilution Techniques and Calculations
Use of the Spectrophotometer
Use of the Dissecting Membrane
Use of the Membrane Filter
Keys to Bacteria
Table of Contents provided by Publisher. All Rights Reserved.

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Customer Reviews

Great June 22, 2011
This textbook is full of interesting experiments in Microbiology with vibrant colors and detail. Book was in the condition that was described so no surprises there. I also received it pretty quickly (didn't realize it until I got it!). I definitely recommend this seller.
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Laboratory Experiments in Microbiology: 5 out of 5 stars based on 1 user reviews.

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