Laboratory Experiments in Microbiology

by Johnson, Ted R.; Case, Christine L.

• ISBN13: 9780805382921
• ISBN10: 0805382925
• Edition: 9th
• Format: Spiral Bound
• Copyright: 2010-01-01
• Publisher: Benjamin Cummings

Summary

KEY MESSAGE: Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case. Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms; Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (endospore, Capsule, Flagella), Morphologic Unknown; Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria; Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods; Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms; Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing; Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens; The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration of Bacteriophages, Plant Viruses; Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique; Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample; Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton; Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria. For all readers interested in microbiology.

Table of Contents

Preface	p. vii
Introduction	p. xi
Color Plates
Staining
Biotechnology
Microbial Metabolism
Eukaryotes
Plate Cultures
Environmental Microbiology
Pathology
Medical Microbiology
Immunology
Growth
Bioremediation
Plate Culture for Enterics
Phototrophic Microorganisms
Unknown Identification: A Photo Quiz
Microscopy	p. 1
Use and Care of the Microscope	p. 3
Examination of Living Microorganisms	p. 13
Staining Methods	p. 23
Preparation of Smears and Simple Staining	p. 25
Negative Staining	p. 31
Gram Staining	p. 35
Acid-Fast Staining	p. 41
Structural Stains (Endospore, Capsule, and Flagella)	p. 47
Morphologic Unknown	p. 57
Cultivation of Bacteria	p. 65
Microbes in the Environment	p. 67
Transfer of Bacteria: Aseptic Technique	p. 75
Isolation of Bacteria by Dilution Techniques	p. 85
Special Media for Isolating Bacteria	p. 93
Microbial Metabolism	p. 97
Carbohydrate Catabolism	p. 99
Fermentation	p. 105
Protein Catabolism, Part 1	p. 113
Protein Catabolism, Part 2	p. 119
Respiration	p. 125
Rapid Identification Methods	p. 129
Microbial Growth	p. 139
Oxygen and the Growth of Bacteria	p. 141
Determination of a Bacterial Growth Curve: The Role of Temperature	p. 149
Biofilms	p. 157
Control of Microbial Growth	p. 165
Physical Methods of Control: Heat	p. 167
Physical Methods of Control: Ultraviolet Radiation	p. 175
Chemical Methods of Control: Disinfectants and Antiseptics	p. 181
Chemical Methods of Control: Antimicrobial Drugs	p. 185
Effectiveness of Hand Scrubbing	p. 193
Microbial Genetics	p. 197
Regulation of Gene Expression	p. 199
Isolation of Bacterial Mutants	p. 207
Transformation of Bacteria	p. 213
DNA Fingerprinting	p. 217
Genetic Engineering	p. 223
Ames Test for Detecting Possible Chemical Carcinogens	p. 233
The Microbial World	p. 239
Unknown Identification and Bergey's Manual	p. 241
Fungi: Yeasts	p. 247
Fungi: Molds	p. 253
Phototrophs: Algae and Cyanobacteria	p. 263
Protozoa	p. 269
Viruses	p. 275
Isolation and Titration of Bacteriophages	p. 277
Plant Viruses	p. 285
Interaction of Microbe and Host	p. 289
Epidemiology	p. 291
Koch's Postulates	p. 299
Immunology	p. 305
Innate Immunity	p. 307
Blood Group Determination: Slide Agglutination	p. 315
Agglutination Reactions: Microtiter Agglutination	p. 321
ELISA Technique	p. 325
Microorganisms and Disease	p. 331
Bacteria of the Skin	p. 333
Bacteria of the Respiratory Tract	p. 337
Bacteria of the Mouth	p. 341
Bacteria of the Gastrointestinal Tract	p. 347
Bacteria of the Urogenital Tract	p. 355
Identification of an Unknown from a Clinical Sample	p. 361
Microbiology and the Environment	p. 365
Microbes in Water: Multiple-Tube Technique	p. 367
Microbes in Water: Membrane Filter Technique	p. 375
Microbes in Food: Contamination	p. 381
Microbes Used in the Production of Foods	p. 387
Microbes in Soil: The Nitrogen and Sulfur Cycles	p. 391
Microbes in Soil: Bioremediation	p. 403
Appendices	p. 409
Pipetting	p. 411
Dilution Techniques and Calculations	p. 415
Use of the Spectrophotometer	p. 419
Graphing	p. 421
Use of the Dissecting Microscope	p. 423
Use of the Membrane Filter	p. 425
Electrophoresis	p. 427
Keys to Bacteria	p. 429
Art and Photography Credits	p. 437
Index	p. 439
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