Laboratory Experiments in Microbiology

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  • Edition: 9th
  • Format: Spiral Bound
  • Copyright: 2010-01-01
  • Publisher: Benjamin Cummings
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KEY MESSAGE: Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case. Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms; Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (endospore, Capsule, Flagella), Morphologic Unknown; Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria; Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods; Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms; Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing; Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens; The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration of Bacteriophages, Plant Viruses; Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique; Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample; Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton; Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria. For all readers interested in microbiology.

Table of Contents

Prefacep. vii
Introductionp. xi
Color Plates
Microbial Metabolism
Plate Cultures
Environmental Microbiology
Medical Microbiology
Plate Culture for Enterics
Phototrophic Microorganisms
Unknown Identification: A Photo Quiz
Microscopyp. 1
Use and Care of the Microscopep. 3
Examination of Living Microorganismsp. 13
Staining Methodsp. 23
Preparation of Smears and Simple Stainingp. 25
Negative Stainingp. 31
Gram Stainingp. 35
Acid-Fast Stainingp. 41
Structural Stains (Endospore, Capsule, and Flagella)p. 47
Morphologic Unknownp. 57
Cultivation of Bacteriap. 65
Microbes in the Environmentp. 67
Transfer of Bacteria: Aseptic Techniquep. 75
Isolation of Bacteria by Dilution Techniquesp. 85
Special Media for Isolating Bacteriap. 93
Microbial Metabolismp. 97
Carbohydrate Catabolismp. 99
Fermentationp. 105
Protein Catabolism, Part 1p. 113
Protein Catabolism, Part 2p. 119
Respirationp. 125
Rapid Identification Methodsp. 129
Microbial Growthp. 139
Oxygen and the Growth of Bacteriap. 141
Determination of a Bacterial Growth Curve: The Role of Temperaturep. 149
Biofilmsp. 157
Control of Microbial Growthp. 165
Physical Methods of Control: Heatp. 167
Physical Methods of Control: Ultraviolet Radiationp. 175
Chemical Methods of Control: Disinfectants and Antisepticsp. 181
Chemical Methods of Control: Antimicrobial Drugsp. 185
Effectiveness of Hand Scrubbingp. 193
Microbial Geneticsp. 197
Regulation of Gene Expressionp. 199
Isolation of Bacterial Mutantsp. 207
Transformation of Bacteriap. 213
DNA Fingerprintingp. 217
Genetic Engineeringp. 223
Ames Test for Detecting Possible Chemical Carcinogensp. 233
The Microbial Worldp. 239
Unknown Identification and Bergey's Manualp. 241
Fungi: Yeastsp. 247
Fungi: Moldsp. 253
Phototrophs: Algae and Cyanobacteriap. 263
Protozoap. 269
Virusesp. 275
Isolation and Titration of Bacteriophagesp. 277
Plant Virusesp. 285
Interaction of Microbe and Hostp. 289
Epidemiologyp. 291
Koch's Postulatesp. 299
Immunologyp. 305
Innate Immunityp. 307
Blood Group Determination: Slide Agglutinationp. 315
Agglutination Reactions: Microtiter Agglutinationp. 321
ELISA Techniquep. 325
Microorganisms and Diseasep. 331
Bacteria of the Skinp. 333
Bacteria of the Respiratory Tractp. 337
Bacteria of the Mouthp. 341
Bacteria of the Gastrointestinal Tractp. 347
Bacteria of the Urogenital Tractp. 355
Identification of an Unknown from a Clinical Samplep. 361
Microbiology and the Environmentp. 365
Microbes in Water: Multiple-Tube Techniquep. 367
Microbes in Water: Membrane Filter Techniquep. 375
Microbes in Food: Contaminationp. 381
Microbes Used in the Production of Foodsp. 387
Microbes in Soil: The Nitrogen and Sulfur Cyclesp. 391
Microbes in Soil: Bioremediationp. 403
Appendicesp. 409
Pipettingp. 411
Dilution Techniques and Calculationsp. 415
Use of the Spectrophotometerp. 419
Graphingp. 421
Use of the Dissecting Microscopep. 423
Use of the Membrane Filterp. 425
Electrophoresisp. 427
Keys to Bacteriap. 429
Art and Photography Creditsp. 437
Indexp. 439
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