9781284100976

Laboratory Fundamentals of Microbiology

by
  • ISBN13:

    9781284100976

  • ISBN10:

    1284100979

  • Edition: 11th
  • Format: Paperback w/ Access Code
  • Copyright: 2017-08-14
  • Publisher: Jones & Bartlett Learning

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Supplemental Materials

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Table of Contents

PartI Laboratory Safety
Lab1 Safety overview: Biohazards
Lab2 Best practices in safety
Lab3 Student safety contract
PartII Laboratory Techniques and Skills
Lab4 Culture transfer techniques
Lab5 Pure culture techniques
Lab6 Pour plate technique
Lab7 Solution Transfer
Lab8 Serial Dilutions
PartIII Microscopy
Lab9 Parts of the microscope
Lab10 Observation of prepared slides
PartIV Bacterial Staining Techniques
Lab11 Preparation of a bacterial smear
Lab12 Simple stain technique
Lab13 The negative stain technique
Lab14 The Gram stain technique
Lab15 Bacterial structures - Spore stain
Lab16 Bacterial structures - Capsule stain
Lab17 Bacterial structures - Bacterial motility, A. Hanging drop B. Motility test agar technique
PartV Viruses and Eukaryotic Microorganisms
Lab18 The effect of bacteriophages on bacteria
Lab19 Plaque formation
Lab20 Identification of bacteriophages from sewage
Lab21 Fungi: Molds
Lab22 Fungi: Yeasts
Lab23 Protozoa and multicellular parasites
PartVI Control of Microorganisms
Lab24 The effect of physical agents on bacteria: Heat tolerance
Lab25 The effect of physical agents on bacteria: Ultraviolet light
Lab26 The effect of physical agents on bacteria: Drying
Lab27 The effect of physical agents on bacteria: Other physical agents, A. Incineration B. Osmotic pressure C. Cold temperature D. Autoclaving
Lab28 The effect of chemical agents on bacteria: Antiseptics and disinfectants
Lab29 The effect of chemical agents on bacteria: Metals
Lab30 The effect of chemical agents on bacteria: Other chemical substances
Lab31 Effect of lysozyme on bacteria
Lab32 Evaluation of disinfectants and antiseptics: Effectiveness of disinfectants on inanimate objects
Lab33 Effectiveness of disinfectants on hand washing and the skin surface
Lab34 Mouthwashes and oral bacteria
Lab35 The effect of antibiotics on bacteria
PartVII Measuring Population Growth
Lab36 Estimating the number of bacterial cells in a population, • The Petroff-Hauser Chamber, • The Standard Plate Count
Lab37 Microbial growth: Analysis of a bacterial growth curve, • Bacterial growth dynamics, • Plotting a growth curve and determining the generation time
PartVIII Medical Microbiology
Lab38 The genus Mycobacterium: Colony and cellular morphology
Lab39 The genus Mycobacterium: Acid-fast stain technique
Lab40 The genus Mycobacterium: Cold acid-fast stain technique
Lab41 The genus Streptococcus: Streptococci from the upper respiratory tract
Lab42 The genus Streptococcus: Streptococci from the oral cavity
Lab43 The genus Neisseria
Lab44 The genus Staphylococcus: Isolation of Staphylococci
Lab45 The genus Staphylococcus: Differentiation between Staphylococcal species
Lab46 The enteric bacteria: Isolation of enteric bacteria
Lab47 The enteric bacteria: Differentiation of enteric bacteria on TSI agar
Lab48 The enteric bacteria: The IMViC series, A. Indole test B. Methyl red test C. Voges-Proskauer test D. Citrate test
Lab49 The enteric bacteria: Rapid identification of enteric bacteria
Lab50 The genus Bacillus
Lab51 The genus Clostridium
Lab52 The genus Lactobacillus: Isolation of Lactobacilli
Lab53 The genus Lactobacillus: Caries susceptibility test
PartIX Identification of a Bacterial Unknown
Lab54 Bacterial structural characteristics
Lab55 Bacterial culture characteristics
Lab56 Biochemical characteristics of bacteria: Carbohydrate fermentation
Lab57 Biochemical characteristics of bacteria: Starch digestion
Lab58 Biochemical characteristics of bacteria: Catalase production
Lab59 Biochemical characteristics of bacteria: DNA digestion
Lab60 Biochemical characteristics of bacteria: Hydrogen sulfate production
Lab61 Biochemical characteristics of bacteria: IMViC series
Lab62 Biochemical characteristics of bacteria: Urea digestion
Lab63 Biochemical characteristics of bacteria: Lipid digestion
Lab64 Biochemical characteristics of bacteria: Casein digestion
PartX Bacterial Genetics
Lab65 Bacterial Genetics: Mutations and the Ames test - Gradient plate technique
Lab66 Bacterial Genetics: Mutations and the Ames test - Mutagenic effect of ultraviolet light
Lab67 Bacterial Genetics: Mutations and the Ames test - DNA repair
Lab68 Bacterial Genetics: Mutations and the Ames test - The Ames test
Lab69 Bacterial conjugation
Lab70 Bacterial transformation
PartXI Immunology
Lab71 Serology: Agglutination techniques and blood cell identification - Slide agglutination
Lab72 Serology: Agglutination techniques and blood cell identification - Tube agglutination
Lab73 Serology: Agglutination techniques and blood cell identification - Hemmagglutination: Determination of blood type
Lab74 Serology: Agglutination techniques and blood cell identification - Blood smear
Lab75 ELISA test
PartXII Public Health and Environmental Microbiology
Lab76 A simulated epidemic: Microbial transmission via fomites
Lab77 A microbial hunt
Lab78 Transmission of microorganisms through toilet paper
Lab79 Disinfection of Drinking Water
Lab80 Microbiology of foods: Food preservation with salt and garlic
Lab81 Microbiology of foods: Standard plate count of food products
Lab82 Microbiology of foods: Fermentation of wine and beer
Lab83 Microbiology of milk and dairy products: Standard plate count of milk
Lab84 Microbiology of milk and dairy products: Coliform plate count of milk
Lab85 Microbiology of milk and dairy products: Methylene blue reduction test
Lab86 Microbiology of milk and dairy products: Preparation of cheese
Lab87 Microbiology of milk and dairy products: Preparation of yogurt
Lab88 Microbiology of milk and dairy products: The natural bacterial content of milk
Lab89 Microbiology of water: Presumptive test by the MPN method
Lab90 Microbiology of water: Confirmed test
Lab91 Microbiology of water: Completed test
Lab92 Microbiology of water: Membrane filter technique
Lab93 Microbiology of water: Preparation of a biofilm
Lab94 Microbiology of soil: Isolation of Rhizobium from legume roots
Lab95 Microbiology of soil: Ammonification of soil microorganisms
Lab96 Microbiology of soil: Isolation of Streptomyces from soil
Lab97 Microbiology of soil: Antibiotic production by Streptomyces
Lab98 Microbiology of soil: Plate count of soil bacteria
Lab99 Microbiology of soil: Microbiology ecology in the soil - the Winogradsky column

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