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9781260163483

Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

by ;
  • ISBN13:

    9781260163483

  • ISBN10:

    1260163482

  • Edition: 3rd
  • Format: Spiral Bound
  • Copyright: 2018-01-25
  • Publisher: McGraw Hill
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Table of Contents

Laboratory Overview: Introduction

Laboratory Safety, Equipment, and Materials

Tools of the Laboratory:  Basic Techniques

Ex 1 Use of the Microscope

Ex 2 Aseptic Techniques

Ex 3 Isolation Streak Plate

Eukaryotic Cells and Microorganisms

Ex 4 Microbial Phototrophs: Algae and Cyanobacteria

Ex 5 Protozoans

Ex 6 The Fungi

Ex 7 Bacterial Motility: Hanging Drop Method

Methods for Microscopic Analysis:  Staining

Ex 8 Simple Staining and Smear Preparation

Ex 9 Negative Stain

Ex 10 Capsule Stain

Ex 11 Gram Stain

Ex 12 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun Methods)

Ex 13 Endospore Stain (Schaeffer-Fulton Method)

Methods for Culturing and Diagnosing Microbes:  Media

Ex 14 Blood Agar

Ex 15 Mannitol Salt Agar

Ex 16 Eosin Methylene Blue Agar

Ex 17 MacConkey Agar

Microbial Nutrition, Ecology, and Growth

Ex 18 Chemical Content of Media

Ex 19 Osmotic Pressure and Growth

Ex 20 Oxygen and Growth:  Aerotolerance

Ex 21 Oxygen and Growth:  Catalase

Ex 22 Oxidase

Food and Water Safety

Ex 23 Plate Count

Treatment and Control of Microbial Growth

Ex 24 Killing by Ultraviolet Light

Ex 25 Antimicrobial Susceptibility Testing (Kirby-Bauer Method)

Microbial Genetics and Genetic Engineering

Ex 26 ELISA and Using Nucleotide BLAST (Dry Lab)

Ex 27 Analysis of DNA: Electrophoresis

Ex 28 Gene Transfer:  Transformation

Infectious Diseases

Ex 29 Parasitic Protozoa

Ex 30 Parasitic Worms

Ex 31 Transmission:  Vectors of Disease

Diagnosing Infections

Ex 32 Gel Immunoprecipitation (Immunodiffusion)

Ex 33 Passive (Indirect) Agglutination

Ex 34 Rapid Immunoassays

Ex 35 Hemagglutination Inhibition Assay

Ex 36 EnteroPluri-Test

Environmental Microbiology

Ex 37 Soil Microbiology

Hydrolytic Enzymes and Disease Mechanisms

Ex 38 DNase Test

Ex 39 Gelatin Hydrolysis

Ex 40 Starch Hydrolysis

Ex 41 Casein Hydrolysis

Biochemical Testing and Diagnosis of Disease

Ex 42 Phenol Red Broth

Ex 43 Triple Sugar Iron Agar

Ex 44 Litmus Milk

Ex 45 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate Reactions)

Ex 46 Urease Test

Ex 47 Nitrate Reduction

Putting It All Together

Ex 48 Known Bacteria for Biochemical Testing

Ex 49 Identification of Unknown Bacteria

Index


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