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9780121820954

Methods in Enzymology: Guide to Yeast Genetics and Molecular Biology

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  • ISBN13:

    9780121820954

  • ISBN10:

    0121820955

  • Format: Hardcover
  • Copyright: 1991-01-01
  • Publisher: Academic Pr
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Supplemental Materials

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Summary

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein

Table of Contents

Contributors to Volume 194 xi
Preface xvii
Volumes in Series xix
Process Guide xxxv
Section I. Basic Methods of Yeast Genetics
Getting Started with Yeast
3(18)
Fred Sherman
Micromanipulation and Dissection of Asci
21(17)
Fred Sherman
James Hicks
Mapping Yeast Genes
38(19)
Fred Sherman
Paul Wakem
Positional Mapping of Genes by Chromosome Blotting and Chromosome Fragmentation
57(20)
Sandra L. Gerring
Carla Connelly
Philip Hieter
Assay of Yeast Matting Reaction
77(17)
George F. Sprague, Jr.
Monitoring Meiosis and Sporulation in Saccharomyces cerevisiae
94(16)
Yona Kassir
Giora Simchen
Identifying Sporulation Genes, Visualizing Synaptonemal Complexes, and Large-Scale Spore and Spore Wall Purification
110(22)
Rochelle Easton Esposito
Michael Dresser
Michael Breitenbach
Putting the HO Gene to Work: Practical Uses for Mating-Type Switching
132(14)
Ira Herskowitz
Robert E. Jensen
Spore Enrichment
146(3)
Beth Rockmill
Eric J. Lambie
G. Shirleen Roeder
Analysis and Manipulation of Yeast Mitochondrial Genes
149(20)
Thomas D. Fox
Linda S. Folley
Julio J. Mulero
Thomas W. McMullin
Peter E. Thorsness
Lars O. Hedin
Maria C. Costanzo
Section II. Cloning and Recombinant DNA
DNA of Saccharomyces cerevisiae
169(13)
Peter Philippsen
Agathe Stotz
Christine Scherf
High-Efficiency Transformation of Yeast by Electroporation
182(5)
Daniel M. Becker
Leonard Guarente
Transmission of Plasmid DNA to Yeast by Conjugation with Bacteria
187(8)
Jack A. Heinemann
George F. Sprague, Jr.
Cloning Genes by Complementation in Yeast
195(35)
Mark D. Rose
James R. Broach
Gene Isolation with gt11 System
230(9)
Richard A. Young
Ronald W. Davis
Gene Overexpression in Studies of Saccharomyces cerevisiae
239(12)
Jasper Rine
Preparation of Clone Libraries in Yeast Artificial-Chromosome Vectors
251(22)
David T. Burke
Maynard V. Olson
Section III. Making Mutants
Classical Mutagenesis Techniques
273(8)
Christopher W. Lawrence
Targeting, Disruption, Replacement, and Allele Rescue: Integrative DNA Transformation in Yeast
281(21)
Rodney Rothstein
In Vitro Mutagenesis and Plasmid Shuffling: From Cloned Gene to Mutant Yeast
302(17)
Robert S. Sikorski
Jef D. Boeke
Recovery of Plasmids from Yeast into Escherichia coli: Shuttle Vectors
319(10)
Jeffrey N. Strathern
David R. Higgins
Shuttle Mutagenesis: Bacterial Transposons for Genetic Manipulations in Yeast
329(13)
Merl F. Hoekstra
H. Steven Seifert
Jac Nickoloff
Fred Heffron
Ty Mutagenesis in Saccharomyces cerevisiae
342(20)
David J. Garfinkel
Jeffrey N. Strathern
Transformation of Yeast Directly with Synthetic Oligonucleotides
362(11)
Richard P. Moerschell
Goutam Das
Fred Sherman
Section IV. Biochemistry of Gene Expression
Vectors for Expression of Cloned Genes in Yeast: Regulation, Overproduction, and Underproduction
373(16)
Jane C. Schneider
Leonard Guarente
Vectors for Constitutive and Inducible Gene Expression in Yeast
389(9)
Mark Schena
Didier Picard
Keith R. Yamamoto
Preparation of High Molecular Weight RNA
398(7)
Karl Kohrer
Horst Domdey
Preparation and Analysis of Low Molecular Weight RNAs and Small Ribonucleoproteins
405(10)
Jo Ann Wise
Measurement of mRNA Decay Rates in Saccharomyces cerevisiae
415(8)
Roy Parker
David Herrick
Stuart W. Peltz
Allan Jacobson
Labeling of RNA and Phosphoproteins in Saccharomyces cerevisiae
423(5)
Jonathan R. Warner
Tackling the Protease Problem in Saccharomyces cerevisiae
428(25)
Elizabeth W. Jones
Structural and Functional Analysis of Yeast Ribosomal Proteins
453(24)
H. A. Raue
W. H. Mager
R. J. Planta
High-Expression Vectors with Multiple Cloning Sites for Construction of trpE Fusion Genes: pATH Vectors
477(14)
T. J. Koerner
John E. Hill
Alan M. Myers
Alexander Tzagoloff
Production of Proteins by Secretion from Yeast
491(17)
Donald T. Moir
Lance S. Davidow
Epitope Tagging and Protein Surveillance
508(12)
Peter A. Kolodziej
Richard A. Young
Reverse Biochemistry: Methods and Applications for Synthesizing Yeast Proteins in Vitro
520(16)
Kevin Struhl
In Vitro Protein Synthesis
536(9)
Michael J. Leibowitz
Francis P. Barbone
Denise E. Georgopoulos
RNA Polymerase II Transcription in Vitro
545(5)
Neal F. Lue
Peter M. Flanagan
Raymond J. Kelleher III
Aled M. Edwards
Roger D. Kornberg
Direct Sequence and Footprint Analysis of Yeast DNA by Primer Extension
550(15)
Jon M. Huibregtse
David R. Engelke
Section V. Cell Biology
Immunofluorescence Methods for Yeast
565(37)
John R. Pringle
Alison E. M. Adams
David G. Drubin
Brian K. Haarer
preparation of Yeast Cells for Thin-Section Electron Microscopy
602(6)
Breck Byers
Loretta Goetsch
Immunogold Labeling of Yeast Ultrathin Sections
608(19)
Michael W. Clark
Analysis of Mitochondrial Function and Assembly
627(17)
Michael P. Yaffe
Methods for Studying the Yeast Vacuole
644(18)
Christopher J. Roberts
Christopher K. Raymond
Carl T. Yamashiro
Tom H. Stevens
Analysis of Polypeptide Transit through Yeast Secretory Pathway
662(13)
Alex Franzusoff
Jonathan Rothblatt
Randy Schekman
In Vitro Protein Translocation across Microsomal Membranes of Saccharomyces cerevisiae
675(7)
Pablo D. Garcia
William Hansen
Peter Walter
Analysis of Glycoproteins from Saccharomyces cerevisiae
682(15)
P. Orlean
M. J. Kuranda
C. F. Albright
Yeast Endocytosis Assays
697(13)
Vjekoslav Dulic
Mark Egerton
Ibrahim Elguindi
Susan Raths
Birgit Singer
Howard Riezman
Inducing and Assaying Heat-Shock Response in Saccharomyces cerevisiae
710(7)
Charles M. Nicolet
Elizabeth A. Craig
Nucleolar-Specific Positive Stains for Optical and Electron Microscopy
717(12)
Michael W. Clark
Staining of Actin with Fluorochrome-Conjugated Phalloidin
729(3)
Alison E. M. Adams
John R. Pringle
Staining of Bud Scars and Other Cell Wall Chitin with Calcofluor
732(3)
John R. Pringle
Isolation of Yeast Nuclei
735(14)
John P. Aris
Gunter Blobel
Analysis of Chromosome Segregation in Saccharomyces cerevisiae
749(25)
James H. Shero
Michael Koval
Forrest Spencer
Robert E. Palmer
Philip Hieter
Douglas Koshland
Genetic Screens and Selections for Cell and Nuclear Fusion Mutants
774(21)
Vivian Berlin
Julie A. Brill
Joshua Trueheart
Jef D. Boeke
Gerald R. Fink
Section VI. Fission Yeast
Molecular Genetic Analysis of Fission Yeast Schizosaccharomyces pombe
795(32)
Sergio Moreno
Amar Klar
Paul Nurse
Section VII. Appendix
Genetic and Physical Maps of Saccharomyces cerevisia
827(38)
Robert K. Mortimer
David Schild
C. Rebecca Contopoulou
Jonathan A. Kans
Author Index 865(40)
Subject Index 905

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