Microbiology Experiments : A Health Science Perspective

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  • Edition: 3rd
  • Format: Paperback
  • Copyright: 2000-12-01

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All experiments are correlated to Nester's "Microbiology: A Human Perspective, 3/e, but can be used with any non-majors/allied health microbiology textbook.

Table of Contents

I Basic Microbiology

Introduction to Microbiology

1 Ubiquity of Microorganisms

2 Bright-field Light Microscopy, Including History and Working Principles

3 Microscopic (Light-field and Dark-field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations

Introduction to Staining of Microorganisms

4 Simple Stains: Positive and Negative Stains

5 Multiple and Differential Stains

Introduction to Microbial Growth

6 Pure Culture and Aseptic Technique

7 Defined, Undefined, Selective and Differential Media

8 Quantification of Microorganisms

Introduction to the Environment and Microbial Growth

9 Aerobic and Anaerobic Growth

10 The Effect of Incubation Temperature on Generation Time

Introduction to Control of Microbial Growth

11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time

12 Control of Microbial Growth with Ultraviolet Light

13 Osmotic Pressure, and its Effect on the Rate and Amount of Microbial Growth

14 Antiseptics and Antibiotics

Introduction to Microbial Genetics

15 Selection of Bacterial Mutants Resistant to Antibiotics

16 Transformation: A Form of Genetic Recombination

17 Bacterial Conjugation

18 Gene Regulation: Induction and Catabolite Repression

II The Other Microbial World

Introduction to the Other Microbial World

19 Microscopic Identification of Fungi

20 Parasitology: Protozoa and Helminths

21 Prokaryotic Viruses: Bacteriophage Isolation and Titering

III Microbes and Humans

Introduction to Medical Microbiology

22 Normal Skin Flora

23 Respiratory Microorganisms

24 Identification of Enteric Gram-negative Rods

25 Clinical Unknown Identification

IV Immunology

Introduction to Some Immunological Principles and Techniques

26 Differential White Blood Cell Stains

27 Lysozyme, an Enzymatic Form of Natural Resistance

28 Traditional Agglutination Reactions Employing Microbial and Nonmicrobial Antigens

29 Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test

30 Use of an Enzyme-linked Immunosorbent Assay (ELISA) Test for Coccidioides immitis Identification

V Public Health and the Role of Microbiology in the Prevention and Control of Communicable Diseases

Introduction to the Prevention and Control of Communicable Diseases

31 An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis Identification

32 Epidemiology: A Staphylococcus Carrier Study

33 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques

VI Applications of Biotechnology

Introduction to Biotechnology

34 Identifying DNA with Restriction Enzymes

35 Identification of Bacteria Using the Ribosomal Data Project

VII Projects

Introduction to the Individual Projects

36 Hydrocarbon Degrading Bacteria, Cleaning Up After Oil Spills

37 Luminescent Bacteria: Bacteria That Produce Light

38 Methylotrophs, Organisms Which Grow on One-Carbon Compounds

39 Deinococcus, Bacteria With-Out-Of -This-World Capabilities

I Microorganisms Used in this Manual

II Dilution Problems

III Metric System with Conversions to the English System of Measurement

IV PH Adjustment of Liquid and Agar Growth Media

V Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size

VI Use of the Hemocytometer for Determining the Total Number of Cells in a Liquid Suspension

VII Preparation of Covered Slide Cultures for Study of the Intact Structure of a Mold Thallus

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