Microbiology Lab Manual

by Harley, John

• ISBN13: 9780077292812
• ISBN10: 0077292812
• Edition: 8th
• Format: Spiral Bound
• Copyright: 2010-02-01
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

Laboratory Exercises in Microbiology, 8/e has been prepared to accompanyPrescott's Microbiology, 8e, written by new authors Joanne Willey, Linda Sherwood, and Christopher Woolverton. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology.

Table of Contents

Microscopic Techniques

Bright-Field Light Microscope and Microscopic Measurement of Organisms

The Hanging Drop Slide and Bacterial Motility

Dark-Field Light Microscope

Phase-Contrast Light Microscope

Flourescence Microscope

Bacterial Morphology and Staining

Negative Staining

Smear Preparation and Simple Staining

Gram Stain

Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures

Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)

Capsule Staining

Flagella Staining: West and Difco's SpotTest Methods

Basic Laboratory and Culture Techniques

Sterilization and Microbiological Culture Media Preparation

Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures

Spread-Plate Technique

The Streak-Plate Technique and Different Media

Pour-Plate Technique

Cultivation of Anaerobic Bacteria

Determination of Bacterial Numbers

Biochemical Activities of Bacteria

Carbohydrates I: Fermentation and B-Galactosidase Activity

Carbohydrates II: Triple Sugar Iron Agar Tests

Carbohydrates III: Starch Hydrolysis

Lipids: Lipid Hydrolysis

Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility

Proteins, Amino Acids, and Enzymes II: The IMViC Tests

Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis

Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis

Proteins, Amino Acids, and Enzymes V: Catalase Activity

Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity

Proteins, Amino Acids, and Enzymes VII: Oxidase Test

Proteins, Amino Acids, and Enzymes VIII: Urease Activity

Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test

Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination

Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction

Rapid Multitest Systems

The API® 20E System

The Enterotube® II System

Unknown Identification

Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria

General Unknown

Environmental Factors Affecting Growth of Microorganisms

Temperature

pH

Osmotic Pressure

The Effects of Chemical Agents on Bacteria I: Disinfectants

The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)

Hand Washing, Environmental Sampling, and Microbiological Monitoring

Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods

Environmental and Food Microbiology

Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test

Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM™ Test for Fecal Coliforms

Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers

Enumeration of Soil Microorganisms

Bacterial Count of a Food Product

Examination of Milk for Bacteria

Medical Microbiology

Hemagglutination Reactions: Blood Groups

Isolation of Normal Microbiota from the Human Body

Staphylococci

Pneumococci

Streptococci

Neisseriae

Aerobic and Anaerobic Endospore-Forming Bacteria

Survey of Selected Eucaryotic Microorganisms

Fungi I: Yeasts

Fungi II: Zygomycota, Ascomyceta, and Basidiomyceta

Microbial Genetics and Genomics

Bacterial Mutation

Bacterial Transformation

Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids

Isolation of Genomic DNA from Baker's Yeast Saccharomyces cerevisiae

Isolation and Purification of Genomic DNA from Escherichia coli

Dilutions with Sample Problems

Metric and English Measurement Equivalents

Transmission-Absorbance Table for Spectrophotometry

Logarithms

pH and pH Indicators

Scientific Notation

Identification Charts

Reagents, Solutions, Stains, and Tests

Culture Media

Sources and Maintenance of Microbiological Stock Cultures

Table of Contents provided by Publisher. All Rights Reserved.